| Titles |
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English :
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Preparation Two Specific Stained Antigens for the Detection of Escherichia Coli O157:H7 Infection and Bru Cellosis
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Arabic :
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تحضير اثنين من الأنتجينات الملونة الخاصة لتشخيص عدوى الميكروب القولونى7H:751O ومرض البروسيلا
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| Abstract |
Escherichia coli 015 7 antigen share elements with Brucella smooth strains which cause cross reactivity in serological standard tests for Brucellosis. In infected animals with Escherichia coli O 157:H7 , antibodies against E. coli cross react with the Brucella antigens (Strain 99 Rose Bengal Test). Consequently, Brucellosis is difficult to diagnosis because shared with E. coli 0157:H7. The aim of this study was to elaborate on a method of antigen preparation that is useful in detecting specific antibodies against each bacterial strain without cross reactivity. In this study, sera were examined from animals that are: vaccinated with Brucella vaccines (Rev.1 and S19); natura1ly infected with Escherichia coli 0151:H7 and Brucellosis; and experimentally infected with Escherichia coli 0151:H7, wild field Brucella melitensis, and other E. coli serotypes. All those sera were ex-amined for antibodies against the developed two antigens. Cross reaction disappeared.. E. coli 0157:H7 antibodies failed to react with the modified Brucella antigen and Brucella antibodies failed to react with the colored antigen of E. coli 0151:H7 .In effect, we were able to demon-strate mixed infection and prove the effectively of the developed antigens. This implicitly proves that the developed antigens have a high degree of specificity and sensitivity. They could be a useful tool for clinical diagnosis and epidemiological studies of E. coli 0151:H7 in-fection and Brucellosis in animals with potential use in humans.
Keywords: Bacterial antigens, bacterial cross reaction. Brucellosis, Enterohaemorrhagic Escherichia coli, TEM (Transmission Electron Microscopy)
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| Publication year |
2008
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| Availability location |
6 شارع النور نادى الصيد الدقى ميشيل باخوم سابقا
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| Availability number |
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| Organization Name |
Climate Change Information Center & Renewable Energy & Expert Systems
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| City |
القاهرة
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| serial title |
مؤتمر الشرق الأوسط وشمال أفريقيا لبحوث الثروة الحيوانية
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| Web Page |
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| Author(s) from ARC |
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| Agris Categories |
Extension
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AGROVOC
TERMS |
Antigens.
Brucellosis.
Escherichia coli.
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| Publication Type |
Conference/Workshop
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