عربى
Claes Home Page
Objectives
Achievement
Organizational Structure
CLAES Projects
Expert Systems
online Expert Systems
Publications
Reasearch Staff
Worshops
Collaborating Institutions
intrnal Links
 
Titles
English : Preparation Two Specific Stained Antigens for the Detection of Escherichia Coli O157:H7 Infection and Bru Cellosis
Arabic : تحضير اثنين من الأنتجينات الملونة الخاصة لتشخيص عدوى الميكروب القولونى7H:751O ومرض البروسيلا
Abstract Escherichia coli 015 7 antigen share elements with Brucella smooth strains which cause cross reactivity in serological standard tests for Brucellosis. In infected animals with Escherichia coli O 157:H7 , antibodies against E. coli cross react with the Brucella antigens (Strain 99 Rose Bengal Test). Consequently, Brucellosis is difficult to diagnosis because shared with E. coli 0157:H7. The aim of this study was to elaborate on a method of antigen preparation that is useful in detecting specific antibodies against each bacterial strain without cross reactivity. In this study, sera were examined from animals that are: vaccinated with Brucella vaccines (Rev.1 and S19); natura1ly infected with Escherichia coli 0151:H7 and Brucellosis; and experimentally infected with Escherichia coli 0151:H7, wild field Brucella melitensis, and other E. coli serotypes. All those sera were ex-amined for antibodies against the developed two antigens. Cross reaction disappeared.. E. coli 0157:H7 antibodies failed to react with the modified Brucella antigen and Brucella antibodies failed to react with the colored antigen of E. coli 0151:H7 .In effect, we were able to demon-strate mixed infection and prove the effectively of the developed antigens. This implicitly proves that the developed antigens have a high degree of specificity and sensitivity. They could be a useful tool for clinical diagnosis and epidemiological studies of E. coli 0151:H7 in-fection and Brucellosis in animals with potential use in humans. Keywords: Bacterial antigens, bacterial cross reaction. Brucellosis, Enterohaemorrhagic Escherichia coli, TEM (Transmission Electron Microscopy)
Publication year 2008
Availability location 6 شارع النور نادى الصيد الدقى ميشيل باخوم سابقا
Availability number
Organization Name
    Central Laboratory for Agricultural Expert System (CLAES)
City القاهرة
serial title مؤتمر الشرق الأوسط وشمال أفريقيا لبحوث الثروة الحيوانية
Web Page
Author(s) from ARC
Agris Categories Extension
AGROVOC
TERMS
Antigens. Brucellosis. Escherichia coli.
Publication Type Conference/Workshop

 
Please email your suggestions to management@claes.sci.eg