| Titles |
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English :
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Effect of sperm selection by percoll and swim up techniques on the sex ratio of rabbit offspring
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| Abstract |
This study was conducted to evaluate rabbit the semen characterization before freezing and after thawing. Also, to detelmine the sex ratio of rabbit offspring's after artificial insemination (A1) using semen selected by Percoll or swim up method. Semen was collected from New Zealand white rabbit bucks (n=9) and 45 rabbit does in three groups (15 in each) were inseminated with frozen semen (control, G1), semen selected by 67.5 per cent Percoll (G2) or swim up (G3) methods, respectively.
Semen was processed in tris-buffer extender at a rate of 1:4 Results showed that percentage of progressive motility, livability and intact acrosome spermatozoa decreased (P<0.05) post-dilution, post-equilibration and post-thawing. Using semen selected by Percoll in does A1 resulted in more females (65.8 %) and less males (34.2 %). Using semen selected by swim up method in AI produced more males (75 %) and less females (25 %). Control does produced sex ratio of 51.2 per cent males and 48.8 per cent females. In conclusion, present results showed that, regardless sperm characteristics in post-thawed rabbit semen and fertility results, semen selected by Percoll density at 67.5 per cent and 10 min centrifugation or swim-up can be used for determining the sex ratio of rabbits. This may control the desire to male or female production prior to conception.
KEY WORDS Rabbit, Semen, Percoll, Swim up, Artificial insemination, Sex ratio
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| Publication year |
2014
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| Availability location |
معهد بحوث الانتاج الحيواني- شارع نادي الصيد دقي- جيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
The Ajas THE ASIAN JOURNAL OF ANIMAL SCIENCE
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| ISSN |
0976-8963
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Artificial insemination.
Rabbits.
Semen.
Sex ratio.
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| Proposed Agrovoc |
Percoll;
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| Publication Type |
Journal
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