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English :
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Partial characterization of bacteriocins produced by two strains of lactic acid bacteria, isolated from traditional Egyptian dairy products
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Arabic :
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توصيف للبكتيروسين الناتج من سلالتين من بكتريا حمض اللاكتيك التى تم عزلها من منتجات الالبان المصرية التقليدية
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| Abstract |
This study presents the characterization of strains of lactic acid bacteria (LAB) from traditional Egyptian dairy products. 623 isolates of lactic acid bacteria (LAB) were studied for their antimicrobial activity against taxonomically related microorganisms. Selected LAB were identified by PCR method as Enterococcus faecium IM1and Enterococcus hirae IM1. Partial purified enterocin from tested strains showed antimicrobial activity against different indicators such as: Brochothrix thermosphacta DSMZ, Carnobacterium maltaromaticum CIP 103135T, Carnobacterium piscicola S4312, Enterococcus faecalis JH2, Listeria grayi CLlP12518, Listeria innocua, Listeria ivanovii ATCC, Listeria monocytogenes and Vagococcus penaei CIP109914T but have no effect against Bacillus subtilis, Moellerella wisconsensis, Pseudomonas fluorescens, Salmonella enterica, Salmonella montevideo, Salmonella typhimurium and Serratia liquefaciens CIP103238T. The inhibitory activity was not due to hydrogen peroxide for Enterococcus hirae IM1, but strain Enterococcus faecium IM1 may excrete diverse antimicrobial compounds such as hydrogen peroxide and bacteriocins. Bacteriocins produced by isolated strains were stable between pH 5 and 8 and stable until 100 ºC/ 20 min. Tested strains were free from some of virulence determinant genes. They may be useful as starter cultures and co-cultures.
Consequently, the selected strains can be applied in fermented dairy foods as efficient biopreservatives cultures.
Keywords: Enterococci, enterocins, Listeria ssp.
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| Publication year |
2014
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| Pages |
561-579
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| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
Journal Food and Dairy Science, Mansoura University
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| ISSN |
2090-3650
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| Author(s) from ARC |
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| External authors (outside ARC) |
شادى نبيل الغايش
جامعة كفر الشيخ
نورفات هوناليم
قسم التكنولوجيا الحيوية الصناعية-كلية الزراعة جامعة الملك سونجولا- تايلاند
محسن عبد العزيز زمارة
جامعة كفر الشيخ
ايمان حسين عياد
سابا باشا - جامعة الاسكندرية
ايفون شوازيت
معهد البحوث الزراعية - ناتت - فرنسا
توماس هيرتلا
معهد البحوث الزراعية - ناتت - فرنسا
جون مارك شوبيرت
معهد البحوث الزراعية - ناتت - فرنسا
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AGROVOC
TERMS |
Bacillus subtilis.
Brochothrix thermosphacta.
Carnobacterium piscicola.
Enterococcus hirae.
Lactic acid bacteria.
Listeria monocytogenes.
Microorganisms.
Streptococcus faecalis.
Streptococcus faecium.
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| Proposed Agrovoc |
taxonomically;Carnobacterium maltaromatic;Listeria grayi;Listeria innocua;Listeria ivanovii;Vagococcus penaei;Listeria ssp;enterocins;Enterococci;
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| Publication Type |
Journal
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