| Titles |
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English :
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Impact of replacing egg yolk with lecithin on sperm characteristics, bacterial count and fertilizing ability of cryopreserved buffalo semen
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Arabic :
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العائد من استبدال صفار البيض بالليسيثين الصويا على خصائص الحيوان المنوى و العد البكتيرى و القدرة الاخصابية في حفظ السائل المنوى للجاموس
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| Abstract |
Soy bean lecithin has been used to replace egg yolk in the semen extender. The aim of this study was to evaluate the effect of replacing egg yolk by soy-lecithin in tris extender on freezability and fertilizing capacity of buffalo semen. Semen from five buffalo bulls was frozen in tris extender containing 0.5, 1.0 and 2% soy-lecithin (treatments) or 15% egg yolk (control). Percentage of motility, livability and intact acrosome spermatozoa and also bacterial count was assessed in diluted, post-equilibrated and post-thawed semen. Results revealed insignificant differences in percentage of sperm motility in diluted semen. However, percentages of sperm motility, livability and intact acrosome spermatozoa increased (P<0.05) and bacterial count decreased (P<0.05) in post-equilibrated and post-thawed semen extended with 0.5% soy-lecithin. These results reflected in the highest conception rate (66.7%) of buffalo cows inseminated with semen containing 05% soy-lecithin compared with 53.3% in semen containing egg yolk (control)
In conclusion, egg yolk could be replaced by soy-lecithin in tris-based extender at a level of 0.5% to increase fertilizing capacity of cryopreserved buffalo semen.
Keywords: Buffalo, frozen semen, sperm, bacterial count, fertility.
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| Publication year |
2014
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| Pages |
353-364
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| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| City |
المنصورة
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| serial title |
Journal Animal and Poultry Production, Mansoura University
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| ISSN |
2090-3642
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Bacteria.
Cell counting.
Egg yolk.
Fertility.
Semen.
Water buffaloes.
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| Proposed Agrovoc |
frozen semen;Soy bean lecithin;semen extender;
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| Publication Type |
Journal
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