| Titles |
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English :
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Genetic differentiation of two Egyptian chicken breeds using 15 microsatellite markers
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| Abstract |
Indigenous chicken are believed to possess better natural immunity against common poultry diseases (Mathur et al., 1989). They could be a source of unique alleles and can contribute to the search for genes associated with health and quality traits (Muchadeyi et al., 2007; Roushdy et al., 2008 and 2009). It also has potential ability to identify unique alleles in the breeds or lines studied. Conservation of farm animals including poultry will be important for future designing of sustainable breeding programs (Toro et al., 2006). However, the extent and level of genetic variability within and among the populations is poorly understood. Genetic differentiation of populations could be detected more accurately by using larger number of loci because each locus contains an independent history of the population depending on the amounts of random drift, mutation and migration that have occurred.
Genetic characterization of chicken breeds and population has been based on egg-white protein polymorphisms (Inafuku et al., 1998), blood protein polymorphisms (Okabayashi et al., 1998), DNA fingerprinting (Yamashita et al., 1994.), random amplified polymorphic DNA (RAPD), (Smith et al., 1996; Singh and Sharma, 2002), and microsatellites (Takahashi et al., 1998; Vanhala et al., 1998; Kaiser et al., 2000; Osman et al., 2006).
Microsatellites or Simple sequence repeats (SSR) are sequences made up of a single sequence motif (1 -6 bp) which is repeated many times side-by-side. Until now, microsatellite markers are the most widely used when assessing the genetic diversity, mapping of QTLs, population structure and relationship in and among various populations of indigenous and commercial chickens including Jungle Fowl (Mwacharo et al., 2007)
It is useful to explore genetic diversity within and between breeds or populations to analyze genetic relationships and admixtures and to provide information on evolutionary relationships and parentage within populations.
Moreover, for breeds undergoing conservation, molecular data should be integrated with other information (i.e., adaptative, productive, and reproductive performances extinction probabilities) to guide decision makers.
This work is an extension to work which was done early in 2009 with six microsatellite marker and recommended with extension work .The present study was done using 15 microsatellite markers for genotyping two indigenous chicken breeds sampled from El-Fayoumi Research Station. The aim of the study was to determine the genetic diversity and population structuring of indigenous Egyptian chicken breeds. Information on the diversity of indigenous genetic resources is an essential tool for designing utilization and conservation programs.
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| Publication year |
2011
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| Pages |
149-161
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| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
Proceeding of the 3rd International Conference of Genetic Engineering &Its Applications
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| Author(s) from ARC |
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| External authors (outside ARC) |
محمد احمد السيد
قسم الموارد الوراثية - البنك القومى للجينات
احمد جلال السيد
جامعة عين شمس
احمد العطار
جامعة عين شمس
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| Agris Categories |
Animal husbandry
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AGROVOC
TERMS |
Genetic variation.
Genotypes.
Immunity.
Microsatellites.
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| Proposed Agrovoc |
indigenous chicken breeds;population structuring;
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| Publication Type |
Conference/Workshop
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