Publications

Titles

English :

Effect of hormonal treatment on rabbits ovarians response, normal embryos production and in vitro embryo development post-thawing

Arabic :

تأثير المعاملة الهرمونية على استجابة مبايض الارانب و انتاج الاجنة الطبيعة و تطور الاجنة معمليا بعد الاسالة

Abstract

The effect of eCG dose injection on rabbits ovarian response, normal embryos production and in vitro survival of embryos after vitrification thawing were examined. Forty-one of multiparous New Zealand White (NZW) rabbits (12 month of age, 3.87±0.16 kg average body weight) were used as embryo donors, allotted in three groups. Does of the 1st group (n= 21) were injected intramuscularly with 20 μg GnRH/doe for induction of ovulation and artificial insemination (control). Embryo donors in the 2nd and 3rd groups (n= 10/group) were administered intramuscularly with 20 or 40 eCG IU/kg live weight (superovulation), 68 h before artificial insemination. Immediately prior to insemination, the does were injected intravenously with hCG at 40 IU/kg live weight. All does were slaughtered at 48-50 h after insemination. Embryos were recovered, counted and evaluated for their stage of development, morphological appearance then vitrified Post 48 h storage in liquid nitrogan (LN2) at -196 °C, embryos were thawed and cultured in vitro with Ham's F10 medium supplemented with 200% FCS for two days. Results showed that donors treated with 20 or 40 IU eCG/kg live weight produced significantly (p<0.01) more CLs, haemorrhagic follicles and yielded three to four folds embryos/doe when compared with GnRH (control group). Embryos recovered from does treated with GnRH (control group) reflected a higher fertilization rate (96.6%) than those recovered from does treated with 20 (89.4%) or 40 (84.1%) IU eCG/kg live weight. The percentage of morphological normal embryos recovered from donors injected with 20 or 40 IU eCG/kg live weight (84.9 or 77.0%) was significantly (P<0.05) lower than that obtained from the normally ovulated does (97.4%). Donors treated with 40 IU eCG/kg live weight retard the rate of embryonic development and reduced their diameters more than those injected with 20 IU eCG/kg live weight compared to GnRH (control group). In 40 IU eCG treated donors-vitrified embryos group, only 61 from 147 embryo vitrified-thaw reached blastocysts (41.5%), whereas 112/186 (60.2%) in 20 IU eCG treated does compared to 74/111 (66.7%) in control group. The present study demonstrates that doe rabbits treated with 20 IU eCG/kg live weight provides superior embryos survival rates than those treated with 40 IU eCG/kg live weight for rabbit embryos production manipulation studies and gene bank. Keywords: Hormonal Treatments, Ovarian Response, Rabbit Embryos, Vitrification.

Publication year

2013

Pages

37-46

Availability location

معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة

Availability number

Organization Name

Animal Production Research Institute (APRI)

serial title

Journal of Production & Development

ISSN