| Titles |
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English :
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Progesterone and estrogen supplementation in vitro culture media improve morphogenesis and development gastrulation embryo rabbits
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Arabic :
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تأثير اضافة كلا من هرمونى البروجستيرون و الاستروجين في بيئة الاستزراع على تحسين الشكل المورفلوجى و معدلات نمو اجنة الارانب المحضنة معمليا فى مرحلة ما قبل تكون الجاسترولا
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| Abstract |
The objective of this study was to compare the development competence of in vitro embryo rabbit and gastrula under culture media supplemented with serum pregnancy does rabbit (SP DR).
Eighty four rabbit embryos from 20 does at 6d.p.C; divided into two groups. The 1st group culture with stander media TCM 199 (control).
The 2nd group culture with TCM 199 supplemented with (10% v/v) pregnancy serum rabbit does with the final concentration of progesterone 9ng/ml and 17fJ-estradiol 20pg/ml for three days.
Numerically viability rates were improved insignificantly according to SPDR additive to culture media. The increase rate significantly (P<0.05) in blastocysts dimension (area 74.29%, perimeter 32.80%) and in gastrula dimension (area 82.02%, perimeter 47.24%) at 48h of culture compared with control group. In the other hand, the rate of collapsed embryos elevated in the control group compared with the serum group. Furthermore, the present results were observed that significantly increased (P<0.05) in gastrulation stages after 8 d.p.c and 9 d.p.c, 65% and 100% of embryos treated with SPDR reached to stage 5 to 7 with strong brachyury expression along the entire primitive streak also in notochordal process.
Conclusively, overall progesterone and estrogen play an important role in the development and implantation of pregastrulation embryos rabbit.
Key words: Progesterone, Embryogenesis, Morphogenesis, Gastrulation, In vitro, Development, Rabbit.
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| Publication year |
2014
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| Pages |
35-45
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| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
Egyptian Journal of Rabbit Science
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| ISSN |
1110-2594
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Embryonic development.
In vitro.
Morphogenesis.
Progesterone.
Rabbits.
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| Proposed Agrovoc |
Gastrulation;
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| Publication Type |
Journal
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