| Titles |
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English :
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Trial for production of FMD cell culture vaccine grown in
medium containing serum replacement
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Arabic :
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بعض المحاولات لإنتاج لقاح الحمى القلاعية من فيروس تم تمريره على نسيج خلوي
نمى على وسط غذائي يحتوي على بدائل السيرم
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| Abstract |
The present study was designed in a trial to use serum replacement instead of the new born calf serum in propagation of BHK-12 cell cultures with subsequent reducing the cost of foot and mouth disease (FMD) vaccine production. Batch was supplemented with newborn calf serum while the medium of the second batch was supplemented with serum replacement. FMD virus was propagated 7 passages using BHK-21 cell culture. Both virus titration and complement fixation titer (CF) revealed that propagation of FMD virus in cell culture supplemented with newborn calf serum yields a titre higher than that in case of cells supplemented with serum replacement. Also two batches of FMD inactivated vaccine were prepared from the virus propagated in the two- mentioned cell culture batches. Two groups of susceptible calves were vaccinated with these vaccines. Both of virus neutralization test (VNT) and enzyme linked immunosorbent assay (ELISA) revealed that higher antibody levels were induced in calves vaccinated with the vaccine prepared from cells supplemented with calf serum then those vaccinated with vaccine prepared from cells grown with serum replacement. BHK-21 cell cultures supplement with newborn calf serum is most susceptible for FMD virus propagation yielding higher titer of the virus. Moreover, the growth pattern of the used cell culture was much better when the newborn calf serum was used.
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| Publication year |
2005
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| Pages |
221-226
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| serial title |
4th scientific conference
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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| Publication Type |
Conference/Workshop
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