| Titles |
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English :
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CHEMICAL AND BACTERIOLOGICAL QUALITY OF FARM AND REAIL MILK SAMPLES
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Arabic :
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الجودة الكيماوية و البكتيريولوجية لعينات لبن مزارع ولبن تجزئة
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| Abstract |
The chemical analysis of the fourty tested raw milkn samples were conducted to measure protein, lactose fat, soilds non fat (SNF) and total solids (TS). The bacteriological quality was performed using different cultivation media either general or specific. Some of zoonotic isolated bacteria were identified to the level of the species.
Obtained results showed that there were no significant differences between the farm and the retail samples for protein, lactose or SNF. On the other hand, high significant differences were found with TS and fat. The same trend of these results was found on the level of the examination seasons. Results of the bacteriological quality exhibited high microbial load of the milk samples appeared on the tryptone glucose extract agar (TGEA) as a general medium either in spring or summer months. Data showed that summer months illustrated higher count with the five cultivation media than those of spring. In case of Salmonella Shigella agar (SSA) medium, some of the retail sample of March showed no count , while in case of using Pseudomonas Aeromonoas selective agar (GSPA) and MacConkey agar (MacCA) media some of the farm samples exhibited negative results in August, while the same retail samples showed count of pathogenic bacteria. The same observation was found in samples of autumn 2002 and winter 2003. Inerestingly, coliform bacteria were not found in winter samples. The pathogenic bacteria were also not found in farm samples of October and November using SSA medium. No significance was found between farm and retail samples on the level of the cultivation media, except Mannitol salt phenol red agar (MSPRA) medium, which is specific for Staphylococcus spp. Also, no significance was found using TGEA and SSA. On the other hand, significant differences were found with MSPRA (Staphylococci), GSPA (Pseudomonas) and MacCa (Coliform bacteria). Additioally, no significance was noticed in the interaction between sample and season for either farm or retail samples. For identification of these bacteria, the cultural, microscopical and biochemical characteristics were carried out. Obtained results showed that the three G+ coccoid-shaped bacteria were belonging to the genus Staphylococcus (Micrococcaceae) and were closely related to Staphylococcus epidermis (114 F5SP), Staphylococcus aureus such as 81 R3A and Staphylococcus aureus like isolate No. 60 R4W.
For the G- short rods, two isolates were found related to the members of the genus Pseudomonas (Pseudomonaceae) suck like Pseudomonas pseudomallei (91 R5SP) and Pseudomonas mallei (92 R5SP). The other three G- short rods were found in relation to the members of Enterobacteriaceae and particularly to the genus Proteus. They were closely related to Proteus mirabilis (35 F2A), Proteus vulgaris l (46 R2W) and Proteus mxyofacience (105 F4S).
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| Publication year |
2006
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| Pages |
431-446
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| Organization Name |
Food Technology Research Institute (FTRI)
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| serial title |
The 8th Sci. Vet. Med. Zag., Conference,
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| Author(s) from ARC |
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| External authors (outside ARC) |
حسين عبدالله الفضالي
قسم الميكروبيولوجي – كلية الزراعة – جامعة المنصورة.
أحمد محمد عبدالجواد الجمل
المعمل الإقليمي بالمنصورة – قسم الميكروبيولوجي– معهد بحوث صحة الحيوان – مركز البحوث الزراعية.
محمد منصور قاسم
قسم الميكروبيولوجي – كلية الزراعة – جامعة المنصورة.
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| Publication Type |
Conference/Workshop
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