| Titles |
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English :
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Viability, enzymatic activity and penetrating ability of spermatozoa into she-camel cervical mucous as affected by different extenders
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| Abstract |
Five males camels at 5 to 10 years of age and 500 - 600 kg body weight, were used. Semen was collected, evaluated and extended with 7 extenders (glucose-yolk-citrate: GYC, fructose-yolk-citrate: FYC, lactase-yolk-citrate : L YC, sucrose -yolk-citrate: SYC, tris-yolk-fructose : TYF, skim- cow -milk: SCM and skim- camel -milk: SLM).
The extended semen was then incubated at 37°C for up to 12 hours. After each incubation time (0, 1, 2, 4, 6, 8, 10, 12 hours), the percentage of motile spermatozoa, dead spermatozoa, sperm abnormalities and acrosomal damage of the camel spermatozoa were record. Aspartate-
aminotransferase (AST), alanine - aminotransferase (ALT) and alkaline phosphatase (ALP) enzymes activities, were also determined. The penetrating ability of spermatozoa into she-camel cervical mucous with the different extenders, during incubation at 37°C for up to 4 hours was also assessed.
The result showed that, the extended camel semen with each of
LYC, SYC, TYF, SCM and SLM extenders increased significantly (P < 0.05) the percentage of sperm motility decreased significantly (P < 0.05) percentage of dead spermatozoa, sperm abnormalities and acrosomal damage of spermatozoa and activities of AST, ALT and ALP enzymes as compared with GYC and FYC extenders, during incubation at 37°C for up to 12 hours. The highest (P < 0.05) percentage of sperm motility was recorded with the extended semen with TYF extender and the lowest (P< 0.05) value was recorded with FYC extender. The highest (P<0.05) percentages of dead spermatozoa, sperm abnormalitites, acrosomal damage and activities of AST, ALT and ALP enzymes were recorded with the extended semen with FYC extender and the lowest (P < 0.05) value. TYF extender.
The advancement of incubation time at 37°C for up to 12 hours decreased significantly (P < 0.05) the percentage of motile spermatozoa, while increased significantly (P<0.05) the percentage of dead spermatozoa, sperm abnormalities, acrosomal damage of spermatozoa and the amount of AST, ALT and ALP enzymes released into the extracllular fluid of the extended camel semen with the all different extenders. The penetrating ability of the extended spermatozoa with TYF, SYC, SCM, SLM and LYC extenders into she-camel cervical mucous was it1sipu,ficantly better than GYC or FYC extender. While, the penetration score of spermatozoa was significantly (P<0.05) decreased with the advancement time of incubation at 37°C with the different extenders.
Key words: Camel semen, extenders, enzymes, incubation, penetration score
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| Publication year |
2011
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| Pages |
255-263
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| Availability location |
معهد بحوث الانتاج الحيوانى-شارع نادى الصيد- الدقى- الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
Journal of Camel Practice and Research
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| ISSN |
0971-6777
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| Author(s) from ARC |
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| External authors (outside ARC) |
جمال محمد عبسى
طب بيطرى- جامعة قناة السويس
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Enzymes.
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| Proposed Agrovoc |
penetration score;extenders;Camel semen;acrosomal damage;sperm abnormalities;dead spermatozoa;motile spermatozoa;
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| Publication Type |
Journal
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