| Titles |
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English :
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Esterase activity in deltamethrin resistant strain of pink bollworm Pectinophora gossypiella (saunders).
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Arabic :
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نشاط إلإنزيمات الإستريزية في سلالة دودة اللوز القرنفلية Pectinophora gossypiella (Saunders) المقاومة لمبيد الدلتامثرين
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| Abstract |
The development of resistance to the pyrethroid insecticide deltamethrin and the biochemical activity of esterases in the resistant strain were measured in the pink bollworm Pectinophora gossypiella (Saunders). Data indicated a gradual progression of resistance during most of the selection process. The resistance ratio increased and reached 108.02-fold in G37. The highest level of resistance, 289.93-fold, occurred in G55. Regarding the biochemical activities in the deltamethrin resistant strain, -esterase activity increased significantly after eight generations of selection as compared with the susceptible strain; the β-esterase activity fluctuated across the different selected generations and ranged between 13.73 to 18.16 µg β-naphthyl/min/g larval weight during the generations of 41 to 55. The electrophoretic patterns of the -esterase enzyme of the deltamethrin resistant strain differed from those of the susceptible strain. Two bands were present in the susceptible strain while between two to four bands in all the tested generations of the deltamethrin resistant strain with different densities. In the case β-esterase isozyme, one band appeared in the susceptible strain, G33, G41, G46 and G48 with different densities; two bands with different densities in G36 and G43; and three bands with different densities in G53 and G55. The induction of newly synthesized bands in selected generations could be a result of genes as a response to the resistance. The occurrence of new bands is a directory of the changes between the susceptible and resistant strains. These data are essential to discuss the mechanism of resistance of such strain.
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| Publication year |
2010
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| Pages |
1-11
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| Organization Name |
Plant Protection Research Institute (PPRI)
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| City |
القاهرة
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| serial title |
النشرة البحثية- كلية زراعة
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| Author(s) from ARC |
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| External authors (outside ARC) |
محمد السعيد الزميتي
كلية زراعة – جامعة عين شمس
علاء الدين بيومي
كلية زراعة – جامعة عين شمس
خالد عبد العزيز سليمان
كلية زراعة – جامعة عين شمس
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| Agris Categories |
Pests of plants
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AGROVOC
TERMS |
Deltamethrin.
Esterases.
Pectinophora gossypiella.
Selection.
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| Publication Type |
Journal
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