| Titles |
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English :
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Biochemical and Histopathological aspects of resistant development in pink bollworm Pectinophora gossypiella (saund.) to deltamehrin and Dipel 2x
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Arabic :
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المظاهر البيوكيميائية والنسيجية المرضية لتطور مقاومة دودة الوز القرنفلية
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| Abstract |
Study the resistance development of the 4th instar larvae of pink bollworm Pectinophora gossypiella (saund.) against deltamethrin and Dipel 2x was carried out under laboratory conditions throughout the selection pressure until 14 generations. Mechanism of development of resistance was studied at biological, biochemical and histopathological levels. Biologically, the calculated midpoint toxicity values, (LC50), indicated that the resistance to deltamethrin increased to 215.11-fold while the resistance to Dipel 2x, attained 16-fold based on the susceptible strain after 14 generations of selection. At biochemical level, detoxication enzymes assay revealed that activity of glutathione S-transferase was higher in all selected generations than susceptible strain. Regarding phosphatases activity in Dipel 2x –resistant strain, acid phosphatase increased than susceptible strain. Also, the activity of alkaline phosphatase was significantly decreased in parent, G3, G7 and G14 in deltamethrin resistant strain while the activity of acid phosphatase was not affected except at G10 when it was increased significantly comparing with the control value. On the other hand, study the protein electrophoresis in the midgut of resistance and susceptible larvae revealed that there are new bands appeared in the resistant strains of both compounds which not present in susceptible strain. Considering the histopathological effects, the obtained results showed histological alterations, i.e. thickness of epithelium cells of the larval midgut of both deltamethrin and Dipel 2x resistant strain
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| Publication year |
2006
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| Pages |
271-278
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| Organization Name |
Plant Protection Research Institute (PPRI)
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| City |
القاهرة
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| serial title |
حوليات العلوم الزراعية (المؤتمر العاشر لبحوث التنمية الزراعية)
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| ISSN |
0570-1783
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| Author(s) from ARC |
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| External authors (outside ARC) |
علاء الدين بيومي
كلية زراعة – جامعة عين شمس
محمد السعيد صالح الزميتى
كلية زراعة – جامعة عين شمس
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| Agris Categories |
Pests of plants
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AGROVOC
TERMS |
Deltamethrin.
Pectinophora gossypiella.
Resistance to chemicals.
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| Publication Type |
Conference/Workshop
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