| Titles |
|
English :
|
Effect of melatonin on in vitro development of morula rabbit embryos
|
|
| Abstract |
This study aimed to investigate the effect of melatonin supplementation at different levels, in culture medium, on rabbit embryo development. Embryos were recovered from nulliparous Red Baladi rabbit does by laparotomy technique 72 hours post-insemination. Normal morulae were cultured till the stage of hatched blastocysts in the following four culture media; (i) control (TCM-199 + 20% fetal bovine serum), (ii) control + 10-9M melatonin, (iii) control + 10-6M melatonin and (iv) control + 10-3M melatonin. The high level of melatonin at 10-3M significantly increased the in vitro development of embryos (92% for 10-3M vs. 84% for 10-6M and 76% for 10-9M and control, P<0.05). However, the addition of melatonin with different levels did not improve the hatchability rate of these embryos (16-30% vs. 52% for melatonin groups vs. control, P<0.05). Results show that adding melatonin at 10-3M to morula rabbit embryos enhances their development, while 10-6M melatonin slightly increased blastocyst rate and 10-9M had no effect on embryo development. This effect strongly suggests a physiological role of melatonin in embryo protection.
Key words: Melatonin, In vitro development, embryos, rabbits.
|
| Publication year |
2012
|
| Pages |
331-334
|
| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة
|
| Availability number |
|
| Organization Name |
Animal Production Research Institute (APRI)
|
| City |
شرم الشيخ
|
| serial title |
10 th World Rabbit Congress
|
| Author(s) from ARC |
|
| External authors (outside ARC) |
جمال محمد كامل محيسن
جامعة القاهرة
|
| Agris Categories |
Animal physiology - Nutrition
|
AGROVOC
TERMS |
Melatonin.
Rabbits.
|
| Proposed Agrovoc |
embryos;In vitro development;hatched blastocysts;Normal morulae;hatchability rate;blastocyst rate;
|
| Publication Type |
Conference/Workshop
|