| Titles |
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English :
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Enhancement the fermentation of lactose – whey using recombinants between lactobacillus and tetragenococcus
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Arabic :
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تحسين خواص البكتيريا اللكتوباسيلا في تخمير الشرش المملح عن طريق احداث تغيرات وراثيه في سلالات اللكتوباسيلا مع سلالات اخري مقاومه للملح
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| Abstract |
In this study adapted isolates of Lactobacillus, as well as, Lactobacillus –Tetragenococcus transconjugants were used in bioconverting lactose whey via lactic acid to overcome the disposal problems of whey resulted from cheese industry. Conjugation was used in this study as a mechanism of DNA transfer to release a variety of lactobacillus recombinants. Eight antibiotics were used for genetic marking lactobacillus strains to be used in mating experiments as a selectable markers for isolating transconjugants. The results revealed that L14, L34 and L19 strains were adapted from 4 % up to 7 % NaCl, whereas strain L44 adapted from 5% up to7.5 % NaCl, in addition, L56 adapted from 7 % up to 10 %NaCl. This indicated that 10% NaCl was a top level at which Lactobacillus strains could tolerated. After 30 days the isolates of L34 were stable, whereas the isolates of L14 and L19 strains were unstable because they could not tolerate the higher concentration of NaCl reached, their tolerance was reduced from 7% to 6 %, as well as L56 was reached to 9% than 10% NaCl, but L44 was reached to 6.5 % than 7.5 % NaCl. Treatment of Tetragenococuus halophilus with different levels of temperature has been shown to result in the elimination of chloramphenicol 2 2 7 resistance genes at 35Co, as well as, chloramphenicol, erythromycin, K Cr O and high salt tolerance at 40Co. Transconjugants resulted from the mating between;T14 x L14, T14 x L56 (except Tr28), T14 ,L34, T19 x L36 and T14 x L44 (except Tr44) appeared significant increase in growth percentage. At 7.5% NaCl the trasconjugants resulted from mating between; T14 x L14, T14 x L56 and T14 x L44 appeared significant increase in growth percentage over the mid parents. However, L43 isolates (A1, A2) and L19 isolate (A1) gave biomass yield and conversion efficiency better than their parental strains. Although, transconjugants from the mating between; T14 with L56(I) except for Tr3, Tr5; T14 X L44 (I) except Tr16 and Tr20, as well as, the trasconjugants resulted from mating between T14 XL14 appeared significant increase in lactic acid production. Transconjugant isolates; Tr8,Tr14,Tr17,Tr18,Tr19,Tr20,Tr22,Tr24,Tr25 and Tr40 appeared significant increase in lactic acid yield over the mid parents. Moreover, transconjugant isolates Tr8,Tr13,Tr14,Tr18,Tr22,Tr24,Tr25 and Tr40 appeared significant increase in lactic acid conversion efficiency in relation to the mid parents. On the other hand, the following transconjugants; Tr8, Tr14, Tr18, Tr22, Tr24, Tr25 and Tr40 appeared significant increase over their mid parents in both lactic acid yield and conversion efficiency.
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| Publication year |
2009
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| Pages |
761-775
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| Availability location |
معهد بحوث تكنولوجيا الأغذية -- مركز البحوث الزراعية - الجيزة.
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| Availability number |
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| Organization Name |
Food Technology Research Institute (FTRI)
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| serial title |
Australian Journal of Basic and Applied Sciences
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| Author(s) from ARC |
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| Agris Categories |
Food science and technology
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AGROVOC
TERMS |
Lactobacillus.
Lactose.
Whey.
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| Publication Type |
Journal
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