| Titles |
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English :
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Immunogenicity of BCG vaccines prepared from different strains.
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Arabic :
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مناعية بعض لقاحات BCG المحضرة من عترات مختلفة
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| Abstract |
Four BCG vaccines from different origins (Danish, French, Canadian and Indian), were studied for their culture characters on different media (Sauton potato, Sauton liquid and Lowenstein-Jensen media), colonial morphology and microscopic appearance. On Sauton potato medium, the complete culture growth was appeared as smooth thin pellicle at 19 days for Danish vaccine while it was smooth thick pellicle at 16, 18 and 20 days for French, Canadian and Indian vaccines respectively. The harvested growth on Sauton liquid medium after 2 successive passages were 0.9 grams at 8 and 7 days intervals for Danish vaccine, 1.3 grams at 7 and 6 days intervals for French vaccine, 1.1 grams at 7 and 7 days intervals for Canadian vaccine and 1.0 grams at 8 and 6 days intervals for Indian vaccine. The growth on Lowenstein-Jensen medium for Danish, Canadian and Indian vaccines appeared moderate scattered minute colonies at 17, 18 and 16 days respectively, while it appeared as heavy scattered minute colonies at 15 days for French vaccine.
The results of the cell mediated immune (CMI) response assessed by lymphocyte proliferation assay recognized that the values in vaccinated increased after 4 days and reach the top 18 days then decline at 32 days post vaccination in different ranges and levels. The higher values noted in vaccinated groups rather than non-vaccinated.
The average body weight in all guinea pig groups that were vaccinated with Danish, French, Canadian and Indian BCG vaccines were increased to (137.0, 142.0, 149.8 and 146.6 grams) respectively after 3 months post vaccination in comparison to control non vaccinated group (111.2 grams).
The skin reactivity results measured by Jensen and tuberculin tests explored that, all groups of guinea pigs that were sensitized with different BCG vaccines developed well clear delayed-type hypersensitivity (DTH).
The viability and heat stability of BCG vaccines at 4° C were 4.7,5.3,4.9 and 4.4 x 106 /ml, while at 37° C were 1.0, 1.0, 0.9 and 1.0 x 106/ml for Danish, French, Canadian and Indian vaccines respectively.
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| Publication year |
2008
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| Pages |
51-64
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| Availability location |
مكتبة معهد بحوث الامصال واللقاحات البيطرية.
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| serial title |
المؤتمر العلمى الخامس لكلية الطب البيطرى بمدينة السادات
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| Author(s) from ARC |
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AGROVOC
TERMS |
Vaccination.
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| Proposed Agrovoc |
BCG vaccines;Chickens immunology;
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| Publication Type |
Conference/Workshop
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