| Titles |
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English :
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The Potential use of Guinea Pigs and Mice as an Alternative to Sheep and Goats for Safety Testing of Peste des Petits Ruminants Live Vaccine
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Arabic :
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إمكانية إستخدام خنازير غينيا والفئران كبديل للأغنام والماعز لاختبار سلامة اللقاح الحى لطاعون المجترات الصغيرة الحى
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| Abstract |
Three Sterile, potent and identified separate batches of the locally manufactured live pestes des petits ruminants virus (PPRV) vaccine were subjected to safety testing in rodents (Guinea pigs and mice) as well as in small ruminants (sheep and goats). For each vaccine batch, three susceptible animals of each of sheep and goats, including one pregnant animal per species were inoculated subcutaneously, each with 1ml of the vaccine containing 5log10 TCID50 of the reconstituted randomly selected, statistically representative samples per batch. Same number & status of animals were held as contact control, inoculated S/C, each with the same volume of normal physiological saline solution as a placebo. Corresponding tests in rodents were done using 10 young and 6 pregnant Guinea pigs as well as 10 unweaned and 6 pregnant mice for each of the three vaccine batches. Five young and 3 pregnant Guinea pigs received an intra-muscular dose of 0.5 ml of 6log10 TCID50/ml per head/batch. The same dose was given intraperitoneally per head of the rest half number of animals. Ten unweaned and 6 pregnant mice received an intraperitoneal dose of 0.1 ml of
6 log10 TCID50/ml per head per batch. A similar number of control rodents were given the same dosing volume of normal physiological saline solution per corresponding routes of inoculations, as a placebo.
All tested small ruminants as well as rodents remained absolutely healthy through out a three weeks observation post inoculateions. Pregnant animals gave birth to normal healthy suckling off springs. Non locating rodents, sacrificed for post-mortem ex-aminations, were absolutely negative to gross pathological findings.
Results obtained would be considered convincing evidence encouraging the orientation to test the locally produced PPRV vaccine safety in rodents as an alternative to sheep and goats. This alternation might save a lot of expenses, time and effort spent in performing one criterion of the quality control integration system.
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| Publication year |
2005
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| Pages |
73-85
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
المنصورة
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| serial title |
مجلة المنصورة الطبية البيطرية
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| ISSN |
1110-7219
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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| Proposed Agrovoc |
المجترات -أمراض;اللقاحات;
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| Publication Type |
Journal
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