| Titles |
|
English :
|
Comparative Evaluation of the Potency of the Attenuated Strain of Peste des Petits Ruminants Virus (Egypt-87) in Vero Cell Culture and in Small Ruminants
|
|
Arabic :
|
مقارنة فاعلية العترة المستضعفة لفيروس لقاح طاعون المجترات الصغيرة فى خلايا فيرو وفى المجترات الصغيرة
|
|
| Abstract |
The object of the present study was to find out a correlation between the potency of the locally produced PPRV vaccine, as estimated in Vero cells and in susceptible sheep. In this respect, studying five batches of the vaccine produced as 1ml lyophilized product / vial / 100 doses revealed that:
1-Infectivity titres per 1ml/vial/100 sheep doses were found to be less than 6 log10 TCID50 on Vero cells, as estimated for the 5 batches of the PPRV vaccine.
2-Five serial ten fold dilutions (10-3-10-7) of batch 1 were used to inoculate (S/C), 5 susceptible sheep groups of 5 heads. Three weeks post inoculation, serum samples were VNT-positive as of 100%, 100%, 60%, 20% and 0% for the 5 respective groups. Corresponding geometric mean neutralizing antibody titres were 80.6, 80.6, 50.8, 8 and 0.
3-Five Susceptible sheep groups of 3 heads were used to test the efficacy of inoculating a dose of 2log10 TCID50 per head, corresponding to the 5 batches /5 groups. Twenty one days post inoculation, serum samples were VNT-positive for each individual animal. Geometric mean antibody titres were 32, 80.6, 80.6, 80.6 and 50.8 for the respective 5 groups.
It was concluded that: 1 Vero TCID50 is equal to 1 sheep ID50. Potency testing of the PPRV vaccine in Vero cells is quite satisfactory and dependable to quality the efficacy of the product. Such a procedure would be considered be considered as a contribution in saving time, effort and money, through carrying out qualification of the PPRV vaccine.
|
| Publication year |
2004
|
| Pages |
175-184
|
| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
|
| City |
المنصورة
|
| serial title |
مجلة المنصورة الطبية البيطرية
|
| ISSN |
1110-7219
|
| Author(s) from ARC |
|
| Agris Categories |
Animal diseases
|
AGROVOC
TERMS |
Ruminants.
|
| Proposed Agrovoc |
verocells;
|
| Publication Type |
Journal
|