| Titles |
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English :
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Detection of Peste des Petits Ruminants and Bovine Ehpermal Fever Viruses by Real Time Pcr
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Arabic :
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الكشف عن فيروس مرض طاعون المجترات الصغيرة و مرض حمي الثلاثة أيام باستخدام اختبار التفاعل المتسلسل لإنزيم البلمرة ( ذو الوقت الحقيقي).
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| Abstract |
In order to test the reliability of real time RT-PCR SYBR green I melting curve analysis of the fusion (F) protein gene for detection of Peste des Petits Ruminants (PPR) virus using seven Saudi field isolate (test), Nigerian 75/1 vaccinal strain (positive control), and kabet’o strain of Rinderpeste (RP) virus (negative control). PPR virus strain and isolated were identified by their distinctive temperature of melting (Tm) ; 87.3°C for field isolates, and 89.3° C for Nigerian 75/1 strain. Also, Real time RT – PCR SYBR Green I melting curve, and Taq Man probe amplification curve were used for detection of Bovine Ephermal Fever (BEF) virus. The two tests quantitatively detected RNA of the virus dilutions between 10-1 and 10-6. The obtained Tm scores of BEF virus were approximately between 77.7°C and 77.9°C.
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| Publication year |
2008
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| Pages |
629-636
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
اسيوط
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| serial title |
المؤتمر العلمي الثالث عشر لكلية الطب البيطري- جامعة اسيوط
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| Author(s) from ARC |
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| External authors (outside ARC) |
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| Agris Categories |
Animal diseases
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| Proposed Agrovoc |
المجترات -أمراض;
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| Publication Type |
Conference/Workshop
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