| Titles |
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English :
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Application of Real Time-PCR for Identification of Sheep PoxVirus Camel Pox Virus and Contagious Ecthyma Virus Isolates.
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Arabic :
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تطبيق استخدام اختبار التفاعل المتسلسل لإنزيم البلمرة (الوقت الحقيقي)في تعريف فيروسات جدري الأغنام وجدري الإبل والتهاب الجلد البثري الوبائي
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| Abstract |
Recently, attention has been directed toward the application of Real time – PCR assays as a rapid and accurate tools for identification of Capripox, Parapox and Orthopox virus that cause devastating diseases in farm animals in the Kingdom of Saudi Arabia.
SYBR Green Real Time PCR assays with primers pairs ;Ks.1 of Capripox viruses, 045 Orf of Parapox viruses and Q Orf of Panparapox viruses were adopted on a panel of Saudi field isolates and reference strains of sheep and goat poxviruses, camel pox virus, reference strains of Lumpy skin disease and vaccinia viruses, and Saudi field isolates of contagious ecthyma (Orf) virus. Capri KS.1 primer set succeeded to amplify all tests DNAs of sheep and goat pox, Camel pox, Lumpy skin disease and vaccinia viruses. With melting curve analysis, temperature of melting (Tm) scored by sheep and goat poxvirus, camel pox virus isolates and their reference strains were relatively identical (between 81.1 and 81.8°C), while Tm scored by Lumpy skin disease and vaccinia virus were 82.1 and 83.2° C respectively no amplification was detected from DNAs of Orf virus isolates tested by Capri – KS.1 primers. However the Orf virus isolates were fairly equivalent amplified with both of 045 Orf primer set (mean Tm score 92.5°C ) and Q Orf primer set (mean Tm score 84°C), as well as with TaqMan Real time-PCR in the presence of Taq Man probe and Q Orf primer set. Otherwise, occasional non-specific amplification of some isolates and strains of Capripox and Orthopox viruses were detected by too late cycle of amplification (>35 cycle) with 045 Orf and Q Orf primer sets.
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| Publication year |
2008
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| Pages |
37-46
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
Giza
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| serial title |
Veterinary medical journal
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| Author(s) from ARC |
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| External authors (outside ARC) |
منصور هاشم عبد الباقي
المعمل المركزي للرقابة على المستحضرات البيطرية
احمد رفعت حبشي
ادارة الثروة الحيوانية وزارة الزراعة - الرياض
محمد احمد سعد
المعمل المركزي للرقابة على المستحضرات البيطرية
محمد ربيع
ادارة المختبرات البيطرية- وزارة الزراعة - الرياض
ابراهيم حسين عبد الرحيم
ادارة المختبرات البيطرية- وزارة الزراعة - الرياض
على بن ناصر الهفوفي
ادارة المختبرات البيطرية- وزارة الزراعة - الرياض
على بن احمد العيسى
ادارة المختبرات البيطرية - وزارة الزارعة الرياض
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| Agris Categories |
Animal diseases
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| Proposed Agrovoc |
الأغنام-أمراض;الأغنام-أمراض;
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| Publication Type |
Journal
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