| Titles |
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English :
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Effect of gossypol on early development of bovine embryo
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Arabic :
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تأثير الجوسيبول على المراحل المبكرة لنمو الجنين في الابقار
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| Abstract |
The purpose of this study was to evaluate the in vitro effect of gossypol on early bovine development with several concentrations that fall within the range of values found in blood plasma and tissue of cows fed cottonseed meal. Two hundreds and thirty excellent and good quality bovine morula were randomly cultured in 0,1,5,10 or 30 g/ ml gossypol acetic acid (GAA) in tissue culture media 199 (TCM 199). Bovine embryo development was assessed at 12-h intervals for 96h. seventy-two percent of embryos developed in 0g/ ml GAA to the hatched blastocyst stage, while 42,18,3 and 0% had comparable development in 1,5,10 and 30 g/ml GAA, respectively. Embryos in 5 g/ml GAA had a delayed development to the blastocyst stage compared to embryos in 1 g/ml GAA. Development time to expanded blastocyst stage was longer for10 g/GAA embryos than 0 and 1g/ml GAA – treated embryos. No embryo cultured in 30 g/ml GAA advanced past the morula stage. Final developmental scores were highest in 0 g/ml GAA (4.06) and lowest for embryos cultured in 10 g/ml GAA (0.44). embryos cultured in higher doses of GAA degenerated sooner than embryo cultured in 0 g/ml GAA. These data show a dose-dependant detrimental action of GAA on early bovine embryo development and suggest a direct action on the embryo itself.
Key words: bovine, embryo, gossypol, in vitro development.
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| Publication year |
2001
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| Pages |
673-679
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| City |
المنصورة
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| serial title |
Journal Agricultural Science Mansoura University
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| Author(s) from ARC |
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| External authors (outside ARC) |
يونج تشانج لن
جامعة اوهايو- الولايات المتحدة الامريكية
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Bovinae.
Embryonic development.
Gossypol.
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| Proposed Agrovoc |
in vitro development;
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| Publication Type |
Journal
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