| Titles |
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English :
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Preparation and evaluation of an inactivated Combined vaccine of bovine Rota, corona viruses and K99 enterotoxigenic Escherichia coli
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Arabic :
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دراسات مناعية لتجهيز وتقييم لقاح جامع ميت لعدوى فيروسات الروتا والكرونا والتهاب القولون المعدي في مصر
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| Abstract |
A formalin inactivated alum gel adjuvanted combined vaccine of bovine rotavirus (BRV), bovine coronavirus (BCV) and k 99 enterotoxigenic Escherichia coli (ETEC) was prepared and evaluated. Ten susceptible calves were vaccinated intramuscularly twice 2 weeks apart using 2 ml/calf. The vaccine was tested for safety in calves and mice and for sterility. Sera of immunized calves showed high antibody levels (4-8 log2 for both BRV and BCV) using serum neutralization test. The ELISA antibody titres were also satisfactory high for the three vaccine components. Passive mouse protection test revealed 80-100% protection against k99 ETEC component. The microagglutination test was also used for evaluation of the potency of k99 ETEC and the titres obtained reached 320 and 1810 at 2 and 8 weeks post vaccination, respectively. It is recommended to prepare the trivalent vaccine locally for control of diarrhea in calves.
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| Publication year |
1999
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| Pages |
957-969
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| Availability location |
ش السكة البيضاء - العباسية -القاهرة
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
القاهرة
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| serial title |
مجلة مركز البحوث الزراعية
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| Author(s) from ARC |
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| External authors (outside ARC) |
محمد سعيد واصل
المعمل المركزي للرقابة على المستحضرات البيطرية
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Bovine rotavirus.
Coronavirus.
Escherichia coli.
Vaccines.
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| Publication Type |
Journal
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