| Titles |
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English :
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Methods for preparation H7 flagellin and polyclonal antibodies for detection of H7 antigens of E/coli O157:H7
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Arabic :
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طريقة جديدة لتحضير H7 السوطى والأجسام المناعية المضادة المتعددة لتشخيص H7 من الميكروب القولوني المعزول
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| Abstract |
E coli of the serotype O157: H7 has recently been isolated from cattle and human fecal samples in Egypt. The aim of this study is to develop an effective method for extraction of flagella from E. coli O157: H7.
Flagella were prepared through the following steps: dissociated by incubation of bacteria, ethanol treatment, supernatant collection and finally precipitated by centrifugation. Flagellar filaments in E. coli O157: H7 were demonstrated by transmission electron microscopy and by PCR. H7 flagellin proteins were shown to be immunogenic. They induced a systemic immune response against those H7 proteins when injected S/C into animals or when given orally.
Polyclonal antiserum raised to H7 flagellin proteins reacted with intact E. coli O157: H7. also, this antiserum reacted strongly with E. coli O11: K+H34 and O156: K-: H23. in effect, the prepared polyclonal sera have the potential to serve as high- quality diagnostic reagent.
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| Publication year |
2008
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| Pages |
119-126
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| Availability location |
ش السكة البيضاء - العباسية -القاهرة
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
القاهرة
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| serial title |
مؤتمر الشرق الأوسط وشمال افريقيا لبحوث الثروة الحيوانية
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| Author(s) from ARC |
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| External authors (outside ARC) |
محمود عبد الواحد رافع
المعمل المركزي للنظم الزراعية الخبيرة
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Antigens.
Polyclonal antibodies.
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| Publication Type |
Conference/Workshop
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