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Titles
English : Preparation two specific stained antigens antigens for the Detection of Escherichia coli 1570: H7 infection and brucellosis
Arabic : تحضير أثنين من الأنتجينات الملونة الخاصة لتشخيص عدوى الميكروب القولونى H7: 751O ومرض البروسيلا
Abstract Escherichia coli O 157 antigen share elements with Brucella smooth strain which cause cross reactivity in serological standard tests for brucellosis. In infected animals which Escherichia coli O 157: H7, antibodies against E. coli cross react with the Brucella antigens (strain 99 Rose Bengal test). Consequently , Brucellosis is difficult to diagnosis because shared with E. coli O 157:H7. the aim of this study was to elaborate on a method of antigen preparation that is useful in detecting specific antibodies against each bacterial strain without cross reactivity. In this study, sera were examined from animals that are: vaccinated with Brucella vaccines (Rev 1 and S19); naturally infected with Escherichia coli O 157:H7 and Brucellosis; and experimentally infected with Escherichia coli O 157:H7, wild field Brucella melitensis, and other E. coli serotypes. All those sera were examined for antibodies against the developed two antigens. Cross reaction disappeared. E. coli O 157:H7 antibodies failed to react with the modified Brucella antigen and Brucella antibodies failed to react with the colored antigen of E. coli O 157:H7. In effect we were able to demonstrate mixed infection and prove the effectively of the developed antigens. This implicity proves that the developed antigens have a high degree of specificity and sensitivity. They could be useful tool for clinical diagnosis and epidemiological studies of E. coli O 157:H7 infection and Brucellosis in animals with potential use in humans.
Publication year 2009
Pages 107-118
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
    Veterinary Serum and Vaccine Research Institute (VSVRI)
City القاهرة
serial title المؤتمر الثانى لبحوث الثروة الحيوانية بمنطقة الشرق الأوسط وشمال افريقية
Author(s) from ARC
External authors (outside ARC)
    محمود عبد الواحد رافع المعمل المركزى للنظم الزراعية الخبيرة
Agris Categories Animal diseases
AGROVOC
TERMS
Antigens. Brucellosis. Escherichia coli.
Publication Type Conference/Workshop

 
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