Titles |
English :
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Preparation two specific stained antigens antigens for the
Detection of Escherichia coli 1570: H7 infection and brucellosis
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Arabic :
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تحضير أثنين من الأنتجينات الملونة الخاصة لتشخيص عدوى الميكروب القولونى H7: 751O ومرض البروسيلا
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Abstract |
Escherichia coli O 157 antigen share elements with Brucella smooth strain which cause cross reactivity in serological standard tests for brucellosis. In infected animals which Escherichia coli O 157: H7, antibodies against E. coli cross react with the Brucella antigens (strain 99 Rose Bengal test). Consequently , Brucellosis is difficult to diagnosis because shared with E. coli O 157:H7. the aim of this study was to elaborate on a method of antigen preparation that is useful in detecting specific antibodies against each bacterial strain without cross reactivity. In this study, sera were examined from animals that are: vaccinated with Brucella vaccines (Rev 1 and S19); naturally infected with Escherichia coli O 157:H7 and Brucellosis; and experimentally infected with Escherichia coli O 157:H7, wild field Brucella melitensis, and other E. coli serotypes. All those sera were examined for antibodies against the developed two antigens. Cross reaction disappeared. E. coli O 157:H7 antibodies failed to react with the modified Brucella antigen and Brucella antibodies failed to react with the colored antigen of E. coli O 157:H7. In effect we were able to demonstrate mixed infection and prove the effectively of the developed antigens. This implicity proves that the developed antigens have a high degree of specificity and sensitivity. They could be useful tool for clinical diagnosis and epidemiological studies of E. coli O 157:H7 infection and Brucellosis in animals with potential use in humans.
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Publication year |
2009
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Pages |
107-118
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Availability location |
القاهرة - ش السكة البيضاء - العباسية
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Availability number |
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Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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City |
القاهرة
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serial title |
المؤتمر الثانى لبحوث الثروة الحيوانية بمنطقة الشرق الأوسط وشمال افريقية
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Author(s) from ARC |
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External authors (outside ARC) |
محمود عبد الواحد رافع
المعمل المركزى للنظم الزراعية الخبيرة
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Agris Categories |
Animal diseases
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AGROVOC TERMS |
Antigens.
Brucellosis.
Escherichia coli.
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Publication Type |
Conference/Workshop
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