| Titles |
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English :
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Biosafety and immunogenicity of brucella abortus strain rb51 in pregnant buffaloes
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Arabic :
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الأمان الحيوى والقدرة المناعية للقاح البروسيلا أبورتس عترة أر.بى 51 في الجاموس العشار
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| Abstract |
To determination of the safety and immunogenicity of brucella abortus strain RB51 vaccine in pregnant buffaloes.twelve pregnant buffalos at mid-pregnancy were used in this study. ten were subcutaneeously vaccinated with 5 x 10 10 CFU of Brucella abortus strain RB51 and two were kept as negative control. Body temperature was daily recorded for the first week, and the animals were observed twice daily for the duration of the study for adverse reaction or abortion. Serum samples from all animals were collected before vaccination and weekly thereafter for serological analysis using Rose Bengal plate, Tube Agglutination, Rivanol tests and Dot-blot Immuno assay. Likewise, weekly vaginal, rectal, nasal and conjunctival swabs were obtained for bacteriological culture to check for envonmental vaccinal organism shedding. At eight weeks post-vaccination, blood was collected for lymphocyte blastogenesis.
No abortion or other clinical signs were seen on any animals at any time of experiments. Brucella abortus strain RB51 was isolated from vaginal swabs of three animals and rectal swab of one animal after 2 weeks post-vaccination and throughout the study. Non of the pregnant buffaloes vaccinated with SRB51 developed antibodies detected by Rose bengal, Tube Agglutination or Rivanol tests but all developed antibodies to SRB51 that reacted in a dot immuno assay, using irradiated SRB51 as antigen. Results of lymphocyte blastogenisis test expressed as delta optical density in animals vaccination with SRB51 yield 0.156 + 0.018 compared with 0.030 in unvaccinated group. It can be concluded that, SRB51 is immunogenic in pregnant buffaloes, resulting in both humoral and cell mediated immune response without subsequent abortion or other generalized infection but shedding of vaccinal organism in feces and vaginal discharges of some vaccinated animals were observed.
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| Publication year |
2003
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| Pages |
921-935
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| Availability location |
ش السكة البيضاء - العباسية-القاهرة
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| serial title |
The role of veterinary medicine for community
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| Author(s) from ARC |
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| External authors (outside ARC) |
كامل محمود عمارة
معهد بحوث الأمصال واللقاحات البيطرية
علاء الدين حسين
معهد بحوث الأمصال واللقاحات البيطرية
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Biosafety.
Brucella.
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| Publication Type |
Conference/Workshop
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