Publications

Titles

English :

Trials for preparation of monovalent inactivated Equine Influenza vaccine

Abstract

Equine influenza virus (A/equi-2/CAIRO-2/2000, H3N8) with egg passage 6 (Ep6) was inactivated by 0.1% formalin (40% formaldehyde) and complete inactivation was achievied after 24 hours at 37oC. Two forms of monovalent equine influenza vaccine were prepared using differnt adjuvants, the first with DEAE-Dextran, while the other with Alhydragel and saponin. Both vaccine forms (freeze dried and liquid one) proved to be safe and potent for horses and G.pigs. The mean HI antibody titres in G.pigs ( three weeks post inoculation) were 1408, 1344, while in horses (six weeks post inoculation) were 640, 480 respectively for both vaccines. The antibody titre in horses vaccinated with both vaccine forms were monitored and persisted at protective level for one year. The keeping qualtiy of the locally prepared EI monovalent inativated vaccine either freeze-dried, reconstituted in DEAE-Dextran or the liquid one were studies and the shelf validity for both vaccines were found to be stable at 4oC for one year, while the freeze dried one can be kept at 20oC for three years

Publication year

2009

Pages

300-312

Availability location

معهد بحوث الأمصال واللقاحات البيطرية-القاهرة - ش السكة البيضاء - العباسية ص ب 131

Availability number

Organization Name

Veterinary Serum and Vaccine Research Institute (VSVRI)

City

رأس سدر

serial title