| Titles |
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English :
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BIOCHEMICAL AND HORMONAL CHANGES IN THE FOLLICULAR FLUID IN CASE OF CYSTIC OVARIAN
DISEASES IN COWS
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| Abstract |
ABSTRACT
The follicular fluid is probably not only a simple transudate of blood, but also a complex restricted component of serum and follicular -synthesized secretion.
Normal follicular fluid collected from El-Basateen and El-Warak abattoir at Cairo province. Immediately after slaughtering and evisceration while ultrasound guided collection of cystic fluid samples in living cows was carried out, with the aid of a 5-7.5 MHZ annular array sector probe with its in vitro fertilization (IVF) attachment. Each sample was centrifuged at 4000 rpm. for 10 minutes to remove cellular debris, the supernatant fluids were stored at - 20 C° until biochemical analysis. Follicular and cystic fluid were analyzed for triglycerides, total cholesterol, HDL-cholesterol, LDL-cholesterol, trace elements (copper, zinc, selenium) and hormonal assay (progesterone oestradiol, Cortisol, T3,T4 and prolactin). Commercial test kits were used for biochemical analysis. Progesterone, oestradiol and Cortisol values showed significant increase in cystic fluid in comparison to normal follicular fluid. While prolactin values recorded significant decrease in cystic fluid than normal one. On the other hand non-significant changes were observed in the levels of triiodethyronine (T3) and thyroxin (T4) between normal and cystic follicular fluid. Analysis of normal and cystic follicular fluid revealed significant increase in values of triglycerides, total cholesterol, HDL-cholesterol as well as LDL-cholesterol in cystic fluid than those recorded in normal follicular fluid. Copper concentration of cystic follicular fluid revealed significant decrease compared with normal fluid. While both zinc and selenium values showed non significant difference between the two groups. Analysis of total proteins revealed significant decrease in values of total proteins in cystic fluid compared to normal follicular fluid. While Alb., glob., A/G ratio, as well as alpha, beta and gamma globulin showed non-significant changes.
The present work clarifies that there were great difference between normal and cystic follicular fluid as increased in total cholesterol, HDL-cholesterol, LDL-cholesterol progesterone and estradiol levels, in cystic fluid compared with normal follicular fluid.
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| Publication year |
2007
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| Availability location |
معهد بحوث التناسليات الحيوانية بالهرم
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| Availability number |
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| Organization Name |
Animal Reproduction Research Institute (ARRI)
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| Author(s) from ARC |
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| External authors (outside ARC) |
ليلى اسماعيل عبد المجيد
كلية طب بيطرى -جامعة القاهرة
مصطفى محمود بشندى
كلية طب بيطرى -جامعة القاهرة
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Cow milk.
Cows.
Dairy cows.
Suckler cows.
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| Publication Type |
Researsh & Applied Activities
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