| Titles |
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English :
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ASSESSMENT OF THE EFFICACY OF SEPHADEX FILTRATION OF BUFFALO SPERMATOZOA FOR CRYOPRESERVATION
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| Abstract |
SUMMARY
In the present study, two experiments were con¬ducted. The first experiment aimed to study the influence of buffalo bull semen filtration through¬out different grades of scphadex columns (G-25, G-50. G-75. G-100 and G-200) on semen quality. Semen samples were collected from four buffalo bulls, diluted 1:20 with Tris buffer, loaded in dif¬ferent grades of sephadex columns and kept for 4-5 minutes at 37«>C Filtered semen samples showed significantly (p<0.0001) higher individual motility (68.75 - 84.38 %) in different grades of scphadex when compared to control samples (63.13%). Percentages of live spermatozoa were found to be higher for filtered samples (71.38 -88.63%) than that of control samples (65.94%). Percentages of major and minor abnormal sper¬matozoa were found to be decreased (2.37 - 4.5% and 1.56 - 5.12%, respectively) significantly (p< 0.0001) than that of control samples (5.56 and 6.31%, respectively). Percentages of spermatozoa with acrosomal defects were found to be signifi¬cantly (p<0.0001) lowered (1.44 - 3.88%) signifi¬cantly (p<0.0001) in comparison with control samples (4.63%). The loss in sperm number (0.67 to 0.99 vs 1.25 X 109 /ml) could be adequately compensated by obtaining higher values of all other important semen quality traits. The use of G-75 sephadex grade is recommended for wider use because it gives a more balanced picture of semen quality compared with other grades stud¬ied.
The second experiment aimed to study the effica¬cy of scphadex G-75 filtration of buffalo sper¬matozoa for cryoprescrvation. Semen samples were collected from the same bulls used in the first experiment. Split samples were diluted 1:20 with Tris buffer, filtered through sephadex G-75 columns and centrifuged at 1500 rpm for 5 min¬utes. The supernatant was discarded and the sedi¬ments were re-diluted and frozen in Obtidyl dilu¬ent (Tris based diluent). Filtration was found to improve semen quality after dilution, freezing and thawing. Post-dilution, filtrated sperm motility
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| Publication year |
2006
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| Pages |
295-307
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| Availability location |
معهد بحوث التناسليات الحيوانية بالهرم
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| Availability number |
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| Organization Name |
Animal Reproduction Research Institute (ARRI)
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Buffalo meat.
Buffalo milk.
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| Proposed Agrovoc |
buffalo diseases;
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| Publication Type |
Researsh & Applied Activities
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