| Titles |
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English :
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Application of Touchdown Enzyme Time Release (Tetr)-Pcr for Diagnosis of Chlamydophila Abortus Infection
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| Abstract |
Abstract
Chlamydophila abortus-DNA was detected using a touchdown enzyme time-release (TETR)-polymerase chain reaction (PCR assay as an improved test for sensitive and rapid diagnosis of abortion in small ruminants. Two hundred and fifty two placentae liver or spleen tissue samples from aborting ewes and goats or aborted lambs and kids in which C. abortus infection was suspectec were examined by TETR-PCR and the results were compared with cell culture. Sixty-five tissue samples were found to be TETR PCR positive while only 56 samples were cell culture-positive. After resolution of discrepant samples with a confirmatory nestec PCR assay, TETR-PCR had a sensitivity of 97% and a specificity of 99.5% while culture had a sensitivity of 84.8% and a specificity of 100%. The analytical sensitivity of the TETR-PCR assay was determined with DNA extracted from 4-fold serial dilution of C abortus B577 culture and found to be 0.25 inclusion-forming unit per PCR. No reduction in the analytical sensitivity was noted whei the assay was tested with mouse liver samples spiked with 4-fold serial dilution of C. abortus B577 culture. No target product was amplified when DNA from Chlamydophila pecorum was tested. TETR-PCR used in this study is a practical, rapid, sensitive anc specific assay that could be used for the detection of C. abortus in infected tissue samples. We recommend the use of this assay as i supplemental diagnostic tool for detection of C. abortus in infected tissue samples. © 2003 Elsevier Science Ltd. All rights reserved.
Keywords: Chlamydophila abortus; Touchdown enzyme time-release (TETR)-PCR; Ewes; Goats; Diagnosis
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| Publication year |
2003
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| Pages |
213-217
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| Availability location |
معهد بحوث التناسليات الحيوانية - 5 شارع حدائق الأهرام - الهرم - الجيزة
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| Availability number |
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| Organization Name |
Animal Reproduction Research Institute (ARRI)
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| Author(s) from ARC |
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| Agris Categories |
Veterinary science and hygiene - General aspects
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| Proposed Agrovoc |
Chlamydophila abortus; Touchdo;
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| Publication Type |
Researsh & Applied Activities
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