| Titles |
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English :
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Efficiency of Follicular Fluid on Buffalo Oocyte Maturation, Fertilization and Embryo Development in Vitro
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| Abstract |
SUMMARY:
This study was conducted to assess the effect of buffalo follicular fluid (BuFF) harvest¬ed from large or small follicles added at different concentrations on in vitro fertilization and developmental capacity of buffalo oocytes . Oocytes were collected from the slaughtered buffaloes ovaries and matured in TCM-199 . BuFF was aspirated from large follicles ( LFF; > 8mm in diameter ) or small follicles (SFF;2 to 5mm in diameter) . After maturation for 24h, oocytes were fertilized in vitro with frozen - thawed heparin treated (100|ig/ml;30 min) spermatozoa and cultured on Hepes-TCM-199 with a monolayer of cumulus cells for 7 days .
In Experiment 1 , the harvested BuFF from large , small sized follicles or pooled follicu¬lar fluid was added to the maturation media . Follicular fluid from large sized follicles im¬proved significantly (P<0.05) fertilization rate (41.82 %) and blastocyst development (14.89%) compared with follicular fluid from small sized follicles (24.19 and 1.92% re¬spectively) or pooled follicular fluid (22.41 and 1.61 % respectively) .
In Experiment 2 , follicular fluid harvested from large sized follicles at concentrations of 0 , 5 , 10 , 20 and 40% (v/v), was added to TCM-199 . Maturation medium that supplement¬ed with 10% follicular fluid improved significantly (P<0.05) normal fertilization rate (35.42%) and blastocyst development (19.15%) compared with higher a BuFF concentration of 40% (11.43 and 3.03 % respectively).
In Experiment 3 , high BuFF concentration (40%) was added to the TCM-199 either from the start of culture or after 8-h or 18-h of culture . Maturation medium supplemented with BuFF 40% 18-h of culture had a similar developmental capacity to the blastocyst stage (12.12%) compared with 10% BuFF (15.56%).
In Experiment 4 , follicular fluid from large sized follicles at a concentration of 10% was added to the maturation media with or without FCS . There was a non significant difference in the fertilization or embryonic developmental rates of oocytes matured in , TCM-199 sup¬plemented with, FF, FCS or both FF and FCS .
In conclusion the results of these experiments suggested that addition of BuFF from large sized buffalo follicles at a concentration of 10% to the maturation media enhanced the normal fertilization rate and buffalo embryo development to the blastocyst stage in vitro.
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| Publication year |
2003
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| Pages |
315-329
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| Availability location |
معهد بحوث التناسليات الحيوانية- 5 شارع حدائق الأهرام - الهرم - الجيزة
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| Availability number |
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| Organization Name |
Animal Reproduction Research Institute (ARRI)
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Fluids.
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| Publication Type |
Researsh & Applied Activities
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