| Titles |
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English :
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Polymerase chain reaction for differentiation of pasteurella multocida isolates from turkeys in comparison to strains incorporating in fowl cholera vaccine .
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Arabic :
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تطبيق تفاعل البلمرة لتحديد بصمة عترات الباستريلا ملتوسيدا المعزولة من الرومى بالمقارنة بالعترات الموجودة فى لقاح كوليرا الطيور
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| Abstract |
In the present study , polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and included in this study . strains included 4 vaccinal reference strains of Pasteurella multocida , CU strain and 4 field isolates of pasteurella multocida isolated from diseased turkeys which were identified biochemically and serologically as A:1, A:3,A3*4 and D:11. the obtained results revealed that revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates . the results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain . this can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer . mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A:1, A:3, D:12 and untypable isolates revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A:1, A:3 and D:12 field strains but it could not be protect mice against untypable isolates .
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| Publication year |
2007
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| Pages |
2
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| Availability location |
(معهد بحوث الامصال واللقاحات البيطرية.
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
بني سويف
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| serial title |
مجلة بني سويف الطبية البيطرية
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| Author(s) from ARC |
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| External authors (outside ARC) |
حنان محمد ابراهيم
المعمل المركزي للرقابة على المستحضرات الحيوية
زينب محمد سرور
معهد بحوث الأمصال واللقاحات البيطرية
سيد ابو السعود
معهد بحوث الأمصال واللقاحات البيطرية
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Fowl cholera.
Pasteurella multocida.
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| Proposed Agrovoc |
polymerase chain reaction;
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| Publication Type |
Journal
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