| Titles |
|
English :
|
Trials for preparation and evaluation of peroxidase and fluorescent isothiocyanate conjugated antisera against equine herpesvirus-1.
|
|
Arabic :
|
محاولات لتحضير و تقييم مصل كشاف بالبيوكسيديز و صبغة الفلورسنت لفيروس الهربس
|
|
| Abstract |
A preliminary study was conducted to prepare a polyclonal antibody against local strain of equine herpsvirus-1 (EHV-1), labeled with horseradish peroxidase and fluorescein isothiocynate. The prepared antiserum in rabbit was conjugated by the most cheaper and easier method (periodate method). It was divided into two parts ; the first one was conjugated with peroxidase which gave strong positive reaction at dilution of 1/2000. The second part was conjugated fluorescin isothiocyanate which give the strongest positive reaction at dilution of 1/4000. Both conjugates were succeeded to show its efficiency and liability for detecting EHV-1 when tested against field- samples within its test proper.
|
| Publication year |
2006
|
| Pages |
7
|
| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
|
| City |
Minufiya
|
| serial title |
Minufiya Vet. Journal
|
| Author(s) from ARC |
|
| Agris Categories |
Animal diseases
|
AGROVOC
TERMS |
Equine herpesvirus.
Immune serum.
Immunofluorescence.
Peroxidases.
Rabbits.
|
| Publication Type |
Journal
|