| Titles |
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English :
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Rapid detection of equine influenza virus by using polymerase chain reaction
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Arabic :
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الكشف السريع لفيروس أنفلونزا الخيول باستخدام اختبار البلمرة المتسلسل
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| Abstract |
Equine influenza virus is a cause of epizootic respiratory. Disease of equine. Thirty nasal swab from horses suffering from respiratory manifestation were tested by reverse transcriptase (RT) PCR using primers specific for HA gene. Primer specificity was tested by using RNA extracted from aminoallantoic fluid infected with local isolate of A / Equi- 2 /Cairo – 2/2000 ( H3 N8). Also virus isolation from previous nasal swabs was performed in specific pathogen free embryonated chicken egg (APF- ECE) and Madin Darby Canine Kidney ( MDCK) cell. The overall viral detection with these methods were 43%, 33% and 26% respectively. RT – PCR proved to be the most sensitive and rapid method for viral detection followed by isolation in ECE and MDK cells
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| Publication year |
2008
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| Pages |
3
|
| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
Zagazig
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| serial title |
Zag. Vet .journal
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| ISSN |
1110-1458
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| Author(s) from ARC |
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| External authors (outside ARC) |
د.نهى عباس حلمي
معهد بحوث الأمصال واللقاحات البيطرية
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Equine influenzavirus.
Horses.
Isolation.
PCR.
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| Publication Type |
Journal
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