Titles |
English :
|
Biocontrol efficacy of some Egyptian microbial isolates against potato tuber moth.
|
Arabic :
|
فاعلية بعض العزلات الميكروبية المصرية لمكافحة فراشة درنات البطاطس
|
|
Abstract |
Bacteria and fungi isolated from diseased and dead larvae and pupa of potato tuber moth (PTM) were evaluated for suppression of Phothorimae operculella , the causal agent of P T M. Nine bacterial and five fungal isolates were highly effective against PTM larval population density and mortality, percentage death in PTM larvae ranged from 20.0 to 100 %. Two fungal and two bacterial isolates that afforded maximum reduction in PTM larvae were identified. The bacterial isolates were identified as. Bacillus thuringiensis and Serratia marcenscens. They gave 86.67 % and 93.33 % mortality respectively. The two fungal isolates were identified as Beauveria bassiana and Metarhizium anisopliae the percentage of death, caused by the two isolates were 86.67 % and 100 % respectively. The median lethal doses (LOso's) and the median lethal times (LTso's) were determined for the four antagonists. It showed that Serratia marcenscens has the highest insecticidal activity ( LC so : 2.4 X 10 6 Spore I ml ) and caused rapid mortality ( LT so 6.4 days ) than Bacillus . thuringiensis ( LC :o : 3.1 X 10 6 Spore / ml ) and ( LT .0 7.7 days ) whereas the fungal isolate Metarhizium anisopliae was more pathogenic (LD so : 1.01 x 10 6 spore / ml) and have a rapid morality (LTso:5.9 days) than Beauveria bassiana . The biocontrol efficacy of various combinations of the four isolates were tested. Application of bacterial mixture of Bacillus thuringiensis and Serratia marcenscens at 1:1 % suppressed Phothorimae operculella effectively and recorded the lowest number of holes / tubes / lowest infestation . and lowest percentage of pupation. Application of mixture of the bacteria Serrafia marcenscens and the fungi MetartUZium anisopliae at a rate 1:3 was more effective and resulted in better suppression of PTM.
|
Publication year |
2006
|
Pages |
4691-4704
|
Availability location |
معهد بحوث وقاية النباتات
|
Availability number |
|
Organization Name |
Plant Protection Research Institute (PPRI)
|
City |
المنصورة
|
serial title |
مجله جامعه المنصورة للعلوم الزراعية
|
ISSN |
1110-0346
|
Author(s) from ARC |
|
External authors (outside ARC) |
زينات كامل محمود
كليه العلوم-جامعه القاهرة
|
Agris Categories |
Protection of plants - General aspects
|
AGROVOC TERMS |
Bacillus thuringiensis.
Beauveria bassiana.
Biological control.
Isolation techniques.
Metarhizium anisopliae.
Serratia.
|
Publication Type |
Journal
|