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Titles
English : Biocontrol efficacy of some Egyptian microbial isolates against potato tuber moth.
Arabic : فاعلية بعض العزلات الميكروبية المصرية لمكافحة فراشة درنات البطاطس
Abstract Bacteria and fungi isolated from diseased and dead larvae and pupa of potato tuber moth (PTM) were evaluated for suppression of Phothorimae operculella , the causal agent of P T M. Nine bacterial and five fungal isolates were highly effective against PTM larval population density and mortality, percentage death in PTM larvae ranged from 20.0 to 100 %. Two fungal and two bacterial isolates that afforded maximum reduction in PTM larvae were identified. The bacterial isolates were identified as. Bacillus thuringiensis and Serratia marcenscens. They gave 86.67 % and 93.33 % mortality respectively. The two fungal isolates were identified as Beauveria bassiana and Metarhizium anisopliae the percentage of death, caused by the two isolates were 86.67 % and 100 % respectively. The median lethal doses (LOso's) and the median lethal times (LTso's) were determined for the four antagonists. It showed that Serratia marcenscens has the highest insecticidal activity ( LC so : 2.4 X 10 6 Spore I ml ) and caused rapid mortality ( LT so 6.4 days ) than Bacillus . thuringiensis ( LC :o : 3.1 X 10 6 Spore / ml ) and ( LT .0 7.7 days ) whereas the fungal isolate Metarhizium anisopliae was more pathogenic (LD so : 1.01 x 10 6 spore / ml) and have a rapid morality (LTso:5.9 days) than Beauveria bassiana . The biocontrol efficacy of various combinations of the four isolates were tested. Application of bacterial mixture of Bacillus thuringiensis and Serratia marcenscens at 1:1 % suppressed Phothorimae operculella effectively and recorded the lowest number of holes / tubes / lowest infestation . and lowest percentage of pupation. Application of mixture of the bacteria Serrafia marcenscens and the fungi MetartUZium anisopliae at a rate 1:3 was more effective and resulted in better suppression of PTM.
Publication year 2006
Pages 4691-4704
Availability location معهد بحوث وقاية النباتات
Availability number
Organization Name
    Plant Protection Research Institute (PPRI)
City المنصورة
serial title مجله جامعه المنصورة للعلوم الزراعية
ISSN 1110-0346
Author(s) from ARC
External authors (outside ARC)
    زينات كامل محمود كليه العلوم-جامعه القاهرة
Agris Categories Protection of plants - General aspects
AGROVOC
TERMS
Bacillus thuringiensis. Beauveria bassiana. Biological control. Isolation techniques. Metarhizium anisopliae. Serratia.
Publication Type Journal

 
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