| Titles |
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English :
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Histochemical assessment of mammary gland capacity pertaining to milk production in buffaloes
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| Abstract |
This work was carried out on five Egyptian buffaloes, at successive stages of lactation. Biopsies were obtained from the mammary gland at three stages of lactation: onset (7-10 ds), peak (the 7th w. ) and late, near to the end of lactation (during involution). Histochemical investigations were executed to determine the mammary alveoli density, densiy of cells per alveolus and the activity of the cells, at these Successive stages of lactation. The highest expression of protein staining was found in the thick walled mammary alveoli coinciding with the greatest secretory activity at 7-l0 d. stage and peak stage of lactation than near to the end of lactation at which the adipose connective tissue was increased. During the lactogenesis from onset to peak of lactation the DNA concentration was greater than during involution (DNA was absent in scveral degraded cells). Numerous loci of Alkaline Phosphatase enzyme (AP) were apparent clearly on the outer surface of the alveoiar secretory cells at onset and peak stages denoting high activity of this enzyme at these two stages. The milk yield at these two stages reached 8.54±0.60 and 10.09±.0.44 kg/head/day, respectively. On contrast, at the late stage (involution of glands) few small alveoli showed only weak staining for AP substrate, while most of sections showed disappeurance of staining associated with AP (negative staining) which reflects the weak enzymatic activity of the cells. The milk yield at this late stage was minimized, only 3.02± 0.72 kg/heed/day.
Keywords: Buffaloes, mammary gland, histochemistry
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| Publication year |
2004
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| Pages |
309-320
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد - الدقى - الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
Egyptian Journal Animal Production
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Mammary glands.
Milk production.
Water buffaloes.
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| Proposed Agrovoc |
histochemistry;Biopsies;alveoli density;
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| Publication Type |
Journal
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