| Titles |
|
English :
|
Effects of different extenders and cryoprotective agents on the frozen rabbit semen quality
|
|
Arabic :
|
تأثير بعض المخففات المختلفة و المواد الواقية من التبريد علي نوعيه السائل المنوي المجمد للأرانب
|
|
| Abstract |
Twenty New- Zealand White (NZW) buck rabbits were used. Semen was collected, evaluated and extended with each of tris- yolk – fructose (TYF), lactose- yolk – citrate (LYC) and sucrose- yolk- citrate ( SYC) extenders. The extended semen was than cooled at 5°C with addition of of glycerol or dimethylsulfoxide (DMSO) at levels of 2.4 and 6%. Semen samples were then frozen in straws. Percentages of post – thawing motility, freezability and acrosomal damage of spermatozoa during thawing – incubation at 37°C for up to 2 hours, were estimated.
The fertility rate and litter size at birth of seventy two NZW doe rabbits artificially inseminated with fresh extended semen or frozen – thawed semen containing 2% glycerol or 4% DMSO, were also assessed.
The results showed that, freezing of rabbit semen extended with SYC extender containing 2% glycerol or 4% DMSO increased significantly (P<0.01) percentages of post – thawing motility and freezability of spermatozoa, however, decreased significantly (P<0.0) percentage of acrosomal damage compared to TYF or LYC extender or other levels of glycerol and DMSO. Thawing – incubation time at 37°C for up to 2 hours had a significant (P<0.01) effect on decreasing the percentages of post- thawing motility and freezability of spermatozoa, however, the percentage of acrosomal damage increased significantly (P<0.01) with the successive time of incubation. The fertility rate of NZW doe rabbits artificially inseminated with fresh extended semen was significantly (P<0.05) higher than frozen-thawed semen added with 2% glycerol or 4% DMSO, while fertility rate with 4% DMSO was insivnificantly higher than 2% glycerol. However, the litter size at birth with different types of frozen/ thawed semen was insignificant.
Keywords: Rabbits, extenders, cryoprotectants, frozen semen, rertility
|
| Publication year |
2002
|
| Pages |
239-251
|
| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة
|
| Availability number |
|
| Organization Name |
Animal Production Research Institute (APRI)
|
| serial title |
3rd Science Conference On Rabbit production in Hot Climates
|
| Author(s) from ARC |
|
| External authors (outside ARC) |
احمد حسن دعادر
جامعة الزقازيق
عبد الحليم الدروانى
جامعة الزقازيق
|
| Agris Categories |
Animal physiology - Reproduction
|
AGROVOC
TERMS |
Cryoprotectants.
Movement.
Rabbits.
Semen.
|
| Proposed Agrovoc |
frozen semen;extenders;rertility;lactose- yolk – citrate;sucrose- yolk- citrate;
|
| Publication Type |
Conference/Workshop
|