| Titles |
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English :
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Semen quality, enzymatic activities and penetrating ability of spermatozoa into SHE-Camel cervical mucus as affected by caffeine addition
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| Abstract |
Twelve sexually mature 6 to 10 years old male dromedary camels of 500 to 600 kg body weight, were used to define the effects of various concentrations of caffeine (0, 2.5, 5 and 10 mM/100 ml) addition to the extended camel semen with two different extenders (tris-yolk fructose and lactose-yolk citrate) on the percentage sperm motility and acrosomal damage., during incubation at 37'C for up to 6 hours. Glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), alkaline phosphatase (ALP) and acid phosphatase (ACP) concentrations were estimated. The penetrating ability of spermatozoa into she-camel cervical mucus was also assessed.
The results showed that the extended camel semen with lactose-yolk citrate had insignificantly higher the percentage of sperm motility and insignificantly lower the percentage of acrosomal damage than tris-yolk fructose extender. The extended camel semen with lactose-yolk citrate had insignificantly lower the amount of GOT and ACP and significantly (p<0.01) lower the amount of GPT and ALP released into the extracellular medium than tris-yolk fructose extender. The 10 mM caffeine added to the extended camel semen increased the percentage of motile spermatozoa significantly (p<0.01) and decreased the percentage of acrosomal damage significantly (p<0.01), amount of GOT, GPT and ALP and decreased the amount of ACP released into the extracellular medium insignificantly. Incubation time at 37°C decreased the percentage of motile spermatozoa significantly (p<0.01), while increased the percentage of acrosomal damage and the amount of GOT, GPT, ALP and ACP released into the extracellular medium significantly (p<0.01). The penetrating ability of spermatozoa improved insignificantly when added with 10 mM caffeine into she-camel cervical mucus.
Keywords: Caffeine, camel, enzymes, extenders, semen
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| Publication year |
2002
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| Pages |
153-161
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى -الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
Journal of Camel Practice and Research Vol. 9 No. 2, P 153-161
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Acrosome.
Caffeine.
Camel meat.
Camels.
Enzyme activity.
Enzymes.
Semen.
Spermatozoa.
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| Publication Type |
Journal
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