| Titles |
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English :
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Evaluation of the fumctional integrity of frozen thawed bovine spermatozoa membrane using hypoosmotic swelling test in relation to different packaging methods
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| Abstract |
Five Friesian bulls were used in the present study. Semen was collected, extended and cooled to 5°C over 1.5-2 hours. Glycerol was added to the cooled semen at a level of 7% and kept at 5°C for 6 hours as equilibration period and then packaged in straws and pellets. The thawed -semen frozen either in straws or as pellets was divided into four parts and diluted with fructose-Na-citrate solution at different levels of osmolarities (50, 100, 200 and 300 mOsmol/L) and then incubated at 37°C for up to 60 minutes. After each incubation time (0, 5, 15,30 and 60 minutes), percentages of post-thawing motility, grade of the progressive sperm motility, intact acrosome, hyaluronidase activity, percentage of spermatozoa with coiled tails and swollen spermatozoa, were estimated.
The results showed that, frozen-thawed bovine semen in straws maintained (P<0.01) higher percentage of post-thawing sperm motility and intact acrosome and insignificantly higher grade of the progressive sperm motility than as pellets. However, leakage of hyaluronidase enzyme into the seminal plasma, percentages of spermatozoa with coiled tails and swollen spermatozoa were significantly (P<0.01) lower in the thawed-frozen spermatozoa in straws than as pellets. The highest (P<0.01) values of the percentage of post-thawing sperm motility, grade of the progressive sperm motility and spermatozoa with intact acrosome either in straws or pellets were recorded with the solutions at osmolarity levels of 200 and 300 mOsmol/L and the lowest (P<0.01) values at 50 and 100 mOsmol/L. Frozen-thawed semen either in straws or pellets and diluted with solutions at osmolarity levels of 200 and 300 mOsmol/L decreased significantly (P<0.01) percentages of spermatozoa with coiled tails, swollen spermatozoa and leakage of hyaluronidase enzyme into the seminal plasma as compared to 50 and 100 mOsmol/L. No significant differences were observed for all the previously measurements between solutions with osmolarity levels of 50 and 100 mOsmol/L The prolongation of thawing-incubation at 37°C from 0 to 60 minutes of the thawed-frozen semen either in straws or pellets decreased significantly (P<0.01) the percentage of post-thawing sperm motility, grade of the progressive sperm motility and spermatozoa with intact acrosome. However, percentages of spermatozoa with coiled tails, swollen spermatozoa and leakage of hyaluronidase enzymes into the seminal plasma increased significantly (P<0.01) with the successive times of incubation.
Key words: bull semen, straws, pellets, hypoosmotic, coiled tails, acrosome.
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| Publication year |
2004
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| Availability location |
معهد بحوث الانتاج الحيوانى- شارع نادى الصيد- الدقى - الجيزة
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| Availability number |
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| Organization Name |
Animal Production Research Institute (APRI)
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| serial title |
3rd International Conference On Animal Poultry & Fish Production And Health In Semi-Arid Areas
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Acrosome.
Bovinae.
Bulls.
Osmotic stress.
Pellets.
Semen.
Shock.
Straw.
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| Proposed Agrovoc |
coiled tails;
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| Publication Type |
Conference/Workshop
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