| Titles |
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English :
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Trials for preparation and evaluation of potent tetanus toxin using simple and economic medium
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Arabic :
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محاولات لتحضير وتقييم سموم التيتانوس عالي القوة العياريةباستخدام مستنبتات غذائية بسيطة واقتصادية
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| Abstract |
In this study, three different formulae of media containing some of the requirements needed for the production of tetanus toxin were used. The first formula was without any amino acids or vitamins which showed the least amount of tetanus toxin produces (5 – 10 Lf / ml). The second formula of medium contained two amino acids which gave a good yield of tetanus toxin (30 – 40 Lf / ml). While the third formula of medium with two amino acids and six vitamins showed a high yield of tetanus toxin (50 – 60 Lf / ml). The first formula was excluded as the toxin producer did not comply the minimum requirements needed for use in production of tetanus toxin and hyperimmune serum. The toxin produces by the second or third formulae was suitable for use but formula III was more complicated and highly expensive. So, it is advisable to use the second formulae which comprised only two amino acids (cystine and tyrosine) as a simple and more economic medium for production of tetanus toxin. The produced toxin showed a good immunogenicity which gave a high titer of antitetanic serum (400 IU / ml) after five injections in horses within a short period of intervals
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| Publication year |
2002
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| Pages |
85-93
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
Beni-suef
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| serial title |
Beni-suef Vet.Med.Jornal
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| Author(s) from ARC |
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| External authors (outside ARC) |
سامي محمد شافعي
المعمل المركزي للرقابة
مجدي محفوظ عوض
معهد بحوث الأمصال واللقاحات البيطرية
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Amino acids.
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| Proposed Agrovoc |
Potent tetanus toxin;
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| Publication Type |
Journal
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