| Titles |
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English :
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Detection of FMD virus using a dot immunoblot and RT-PCR from field samples
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Arabic :
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الكشف عن فيروس مرض الحمى القلاعية بإستخدام طريقتي اللطع المناعي النقطي وتفاعل البلمرة المتسلسل العكسي
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| Abstract |
Dot immunoblot assay (DIA) and reverse transcriptase polymerase chain reaction (RT-PCR) were performed to detect foot and mouth disease virus (FMDV) in field samples. FMDV is identified by DIA as a dark blue colored dot using a procedure based on the principle of enzyme linked immunosorbent assay and 4-chloro-1-naphthol as a substrate. All T.E and 5 of nasal swaps tested positive by DIA. RNA extracted from tongue epithelium (T.E) and nasal swap samples were amplified using RT-PCR and two specific primers to ID gene of FMDV serotype O1. The two primers were designed to amplify DNA fragment about 300 bp in length. All T.E and 6 of nasal swaps demonstrated fragments of about 300 bp in length. The five positive nasal swaps with DIA also tested positive by RT-PCR. DIA and RT-PCR were observed by rapid, specific, inexpensive and sensitive techniques for detection of FMD virus.
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| Publication year |
2004
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| Availability location |
مكتبة كلية الطب البيطري بمشتهر - مكتبة معهد بحوث الأمصال واللقاحات البيطرية
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
بنها
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| serial title |
المؤتمر العلمي الأول لكلية الطب البيطري بمشتهر
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| Author(s) from ARC |
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| External authors (outside ARC) |
حليمة محمد الوطني
معهد بحوث الأمصال واللقاحات البيطرية
عبير طلعت
معهد بحوث الأمصال واللقاحات البيطرية
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| Agris Categories |
Animal diseases
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| Proposed Agrovoc |
Dot Immunoblot Assay;RT-PCR;
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| Publication Type |
Conference/Workshop
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