| Titles |
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English :
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Production and evaluation of Anti rift valley fever (RVF) IgG conjugated with fluorescein
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Arabic :
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انتاج وتقييم أجسام مناعية ضد فيروس حمى الوادي المتصدع واتحادها بالفلورسين
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| Abstract |
Three susceptible sheep were inoculated with RVF antigen mixed with incomplete and complete Freund's adjuvant and without adjuvant in three successive doses. The hyperimmune sera collected from the sheep were tested against standard reference RVF antigen for the presence of specific RVF antibodies using serum neutralization test (SNT), agar gel precipitation test(AGPT)and indirect fluorescent assay (IFA). Separation of RVF immunoglobulins were done uaing affinity chromatography (activated sepharose 4B) followed by conjugation of RVF IgG with fluorescen isothiocyanate at pH 9.6 . The conjugated RVF antibodies were titrated against reference RVF antigen and the titre reached (1:128). The working dilution was used for evaluation of different batches of attenuated and inactivated RVF vaccines in comparison with IFA test using anti-sheep Fitic, the result of both assays proved to be simiar.
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| Publication year |
2001
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية - ش السكة البيضاء - العباسية - القاهرة
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
القاهرة
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| serial title |
Egypt. J.Agric, Res, 79(4), 2001.
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| Author(s) from ARC |
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| External authors (outside ARC) |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Antiaris.
Evaluation.
Fluorescence.
Rift valley fever virus.
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| Publication Type |
Journal
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