| Titles |
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English :
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Trials to increase the sensitivity of ELISA technique using ELISA amplifcation system for the diagnosis of FMD
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| Abstract |
Three cattle calves and five sheep were vaccinated against FMD. The serum samples were collected weekly, titers were estimated using conventional ELISA and amplification ELISA system. The results revealed that the mean antibody titer estimated by amplified ELISA system was higher than that estimated by conventional ELISA. The results concluded the importance of using an accurate and sensitive amplified ELISA technique for detection of vaccinated and suspected animals with FMD virus. In this new enzyme detection method, Iabeled enzyme is used to catalyse the (NADP+) to NAD which activates strictly NAD- dependant redox cycle leading to the formation of a coloured product formazan dye.
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| Publication year |
2003
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| Pages |
417-423
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| Availability location |
معهد بحوث الامصال و اللقاحات البيطرية - القاهرة - ش السكة البيضاء - العباسية
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| Availability number |
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
أسيوط
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| serial title |
J. Egypt Vet. Med . Assoe
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| Author(s) from ARC |
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| External authors (outside ARC) |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Aphthovirus.
Calves.
Cattle.
Elisa.
Sheep.
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| Publication Type |
Journal
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