| Titles |
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English :
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Molecular characterization of Mannhiemia haemolytica extracts and utility in serodiagnosis of pasteurellosis in Egypt.
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Arabic :
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توصيف جزئي لمستخلصات مانهيميا هيموليتيكا وفائدتها في استبيان الإصابة بمرض الباستريلا في مصر.
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| Abstract |
Virulence factors of M.haemolytica (A2) and P.trehaloosi (I4) local isolates of caprine origin, represented by outer membrane proteins (OMP),lipopolysaccharides (LPS), leukotoxin (LKT)and bacterial cell lysate (BCL) as well as OMP of p.multocida (B6) standard strain, were comparatively analysed for their antigenic structure and cross-reactive components, using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western immunoblotting. Main differences among M.haemolytica and pasteurella species (P.trehalosi and p.multocida) were observed in their OMP extracts. A37 KiolDaltons (kDa) antigenic determinant was a M.haemolytica (A2) specific immunoreactive protein subunit. The most unique cross-reacting antigens in OMPs of p.trehalosi (T4) and p.multocida (B6) versus polyclonal anti-M.haemolytica (A2)antiserum were at 66,44,43 and 41 kDa that could be potential Pasteurella-related surface antigens. The major immunodetcted antigenic determinants in all M.haemolytica (A2) extracts analysed were at 100-105,66-68,41-44,28-29 and 16-18kDa. Neither SDS-PAGE nor Western immunoblottine analysis revealed marked M.haemolytica and p.trehalosi local isolates. In the indirect enzyme-linked immunosorbent assay (ELISA), indirect haemagglutination (IHA) and rapid plate agglutination (RPA) tests, all antigenic extracts of M.haemolytica (A2) were proved effective potential candidated for serovar-wide detection of anti-M.haemolytica antibody in livestock sera (cattle,buffalo,sheepand camel). however BCL antigen scored higher value than did the OMP then LKt and LPS.The present work extends the epidemiological analysis related to pasteurellosis in EGYPT and iaitiates a potential for development of rapid ddiagnostic reagents for livestock animals at risk.
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| Publication year |
2004
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| Pages |
321- 343
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| Organization Name |
Veterinary Serum and Vaccine Research Institute (VSVRI)
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| City |
القاهرة
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| serial title |
مجلة الجمعية الطبية البيطرية
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| Department |
Aerobic Bacterial Vaccines Research
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Egypt.
Immunodiagnosis.
Pasteurellosis.
Plant extracts.
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| Publication Type |
Journal
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