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English :
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Effect of some hormonal and chemical additions on semen characteristics and storagability in Rans.
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Arabic :
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تاثير بعض الاضافات الهرمونية و الكيمائية على خواص السائل المنوى و قابليته للتخزين في الكباش .
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| Abstract |
The present study was conducted at Alexandria University Experimental Farm on Rahmani and Barki rams. The aim of this study was to investigate the effects of cAMP, caffeine, FF and steroid hormones (namely progesterone, estradiol-17J3 and testosterone) on the quality, maintenance and storageability of fresh, cooled at 5°C and/or pelleted rams spermatozoa. The effects of different incubation periods at 37°C were also studied.To achieve these objectives three experiments were conducted:
Experiment I was designed to investigate the effects of addition of cAMP, caffeine, FF, progesterone, estradiol-17β and testosterone on the percentages of sperm motility, AR, and dead spermatozoa at different times of incubation at 37°C. In this experiment 6 trails were carried out each aimed to evaluate 4 levels of each tested material at different times post incubation. The incubation times were 0, 60, 120, 180, 240, 360 and 480 minutes.
Experiment II was designed to study the effects of the most desirable dose from caffeine, FF and testosterone on storageability of rams spermatozoa at 5°C. Three different treatments were carried out on a fresh semen: 1-semen + Tris-extender (control); 2-semen + tris-extender + either 15mM caffeine or 15% FF or 40°Ug testosterone, were added pre-cooling, and 3-same as 2 except that materials were added post cooling. Motility, AR and dead sperm were assessed in control, pre-cooling and post-cooling samples.
Experiment III was designed to study the effect of the best dose from caffeine, FF and testosterone on the storageability of ram's spermatozoa in frozen pellets form. Seven different treatments were carried out as following: 1-Semen samples + trisextender (control); 2, 3,4- samples + tris-extender + either caffeine, FF or testosterone; 5, 6 and 7 - as 2, 3 and 4 except that the materials were added Post-thawing. Motility, AR and dead . spermatozoa were evaluated immediately post-collection (control), post-cooling and at 0, 2 and 4 hrs Post-thawing and incubating at 37°C.
All data obtained were analyzed statistically by least square analysis of variance using General Linear Models and Duncan's Multiple Range Test.
Results of this study can be summarized as follows:
Experiment I
. Adding cAMP significantly (P<0.01) affected the percentages of sperm motility, AR and dead spermatozoa. Sperm motility was increased but dead spermatozoa and AR was decreased except when cAMP was added in a concentration of 100 mM. At this level AR increased as compared to control samples. As incubation time was increased for samples with cAMP, the percentages of motile sperm significantly decreased but AR and dead spermatozoa were increased in a time- dependant manner.
Adding caffeine significantly (P<0.01) affected the percentages of sperm motility, AR and dead spermatozoa. Sperm motility and
AR were increased by adding different levels of caffeine as . compared to control without caffeine. Percentages of dead spermatozoa were decreased in caffeine-treated samples.
Adding 10mM caffeine gave the best results with regard to all sperm functions studied. As incubation time increased from zero to 480 min sperm motility were decreased but dead spermatozoa were increased (P<0.01). Percentages of AR showed no definite trend throughout the different incubation times.
3- Adding different levels of FF significantly affected (P<0.01) the percentages of motility, AR and dead spermatozoa throughout the incubation periods. Sperm motility and AR increased but dead spermatozoa decreased by adding the different levels of FF. Adding the higher level of FF (25%) significantly (P<0.05) increased sperm motility and AR than the control or the low levels of FF. It was also found that increasing incubation time significantly (P<0.05) decreased sperm motility percentage, but increased sperm AR and dead spermatozoa.
4- Adding different levels of progesterone and incubating for different periods of times significantly (P<0.01) affected sperm motility, AR and dead spermatozoa percentages. Sperm motility was markedly decreased, and AR was increased in treated samples. Progesterone at low levels (10 and 20Ug/ml) decreased dead spermatozoa as compared to control or the high level of progesterone (40/Ug/ml). As incubation time increased sperm motility significantly (P<0.01) decreased, but sperm AR and dead percentages progressively increased in a time-dependant manner.
5- Adding different levels of estradiol-17β and incubating for . different times significantly (P<0.01) affected the percentages of sperm motility, AR and dead spermatozoa. Different levels of estradiol significantly decreased (P<0.05) sperm motility percentages. Acrosome reaction of sperm increased only with the higher level of estradiol (50ng/ml). Dead spermatozoa decreased with adding the low level of E2 -17β (5ng/ml). As incubation time increased sperm motility significantly (P<0.01) decreased but AR and dead spermatozoa were increased in a time-dependent manner.
6- Adding different levels of testosterone and incubating for different times at 37°C significantly (P<0.05) affected sperm motility, AR and dead spermatozoa percentages. Different concentrations of testosterone increased the percentages of motile spermatozoa and AR but decreased dead cells. As incubation with testosterone at 37°C was increased there were significant (P<0.01) decrease in motile spermatozoa but increases in AR and dead spermatozoa.
Experiment 11
Adding FF at 15% level post-cooling increased (P<0.05) sperm motility as compared to control without FF or adding FF pre-cooling.
the effect of adding caffeine or follicular fluid or testosterone on perm motility seem to be negligible and less than that of FF.
Addition of follicular fluid post-cooling significantly decreased dead permatozoa post-incubation as compared to that for pre-cooling samples. Addition of caffeine post-cooling adversely affected vability of spermatozoa, however adding testosterone had a gative effect. A marked increase in AR was observed in caffeine
treated post-cooling. These results indicate that adding FF at 15% leve1 post-cooling enhanced the sperm function during storgeability by cooling compared with that of caffeine and testosterone.
Experiment III
Adding caffeine or FF or testosterone to ram's semen samples post-pelleting and thawing significantly (P<0.05) increased the percentages of sperm motility but decreased both AR and dead spermatozoa as compared to control or to adding the materials at pre-cooling. Testosterone added post-cooling and thawing caused the best motility and the least dead spermatozoa or AR. Incubation of samples post-thawing at 37°C for different periods of time decrease (P<0.05) the percentage of sperm motility, AR but increased dead spermatozoa. The effects on values of AR and dead spermatozoa were markedly pronounced at two and four hours of incubation.
It can be concluded from the present results that adding caffeine in a dose of 10mM or FF at a level of 25% to fresh semen without incubation achieved the best results with regard to their favorable effect on sperm motility and AR. They also reduced the percentage of dead sperm. Results also indicated that adding FF at 5% level post-cooling to preserve semen by cooling is better than adding caffeine or testosterone. This level of FF enhanced sperm functions than the other two materials. In case of preserving semen y freezing (pelleting) it is advisable, according to the present suits, to add any of the test materials (caffeine, FF and testosterone) post-thawing at 37°C.
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| Publication year |
2003
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| Pages |
172
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| Availability location |
مكتبة معهد بحوث الانتاج الحيوانى- شارع نادى الصيد - الدقى- الجيزة
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| Availability number |
804
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| Organization Name |
Animal Production Research Institute (APRI)
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| Country |
Egypt
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| Department |
Sheep and Goat Research Department
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| Author(s) from ARC |
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| Agris Categories |
Animal physiology - Reproduction
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AGROVOC
TERMS |
Caffeine.
Hormonal control.
Maintenance.
Progesterone.
Quality of life.
Semen.
Sheep.
Spermatozoa.
Steroid hormones.
Storage.
Testosterone.
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| Publication Type |
PhD Thesis
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