| Titles |
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Arabic :
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دراسة مناعية على الأفلاتوكسين
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| Abstract |
The present work studied the effect of aflatoxin on the immune system by the following experiments:
1. Effect of aflatoxin on the level of antibody during vaccination for Newcaste disease as well as resistance to velogenic strain of Newcastel disease virus aftr immunization.
2. Studies interaction between aflatoxin and Candida
(
albicans infection.
3. Studied interaction between aflatoxin and Aspergillus fumigatus infection.
In the first experiment, the one day old chicks have been divided into 8 groups, group 1 and group 8 control received normal giet and group 1 vaccinated while group 8 not vaccinated against NDV. Group 2 received 125 ppb/kg
feed, group 3 received 250 ppb/kg feed, group 4 received 500
ppb/kg feed, group 5 received 1000 ppb/kg feed, group 6
received 250 ppb/kg feed for 3 week and after that received normal feed, and group 7 received 500 ppb/kg for 3 week and after that received normal feed.
Maternal antibodies to New castle were estimated using HI test in sera of chickens, after 2,4 and 6 week, we found
aflatoxin induced decrease in the maternal antibody level and this decrease varies according to the increase of aflatoxin.
The antibody titre were (7.64, 6.60 and 7.00) in group 1, in group 2 were (5.0, 5.86 and 4.5), in group 3 (4.45, 4.5 and 4.4) group 4 were (4.4, 4.5 and 3.4), in group 5
were (4.0, 4.5 and 3.4) while in group 6 were (4.5, 4.57 and 4.6) in group 7 were (4.6, 4.46 and 3.64) and in group 8 no detected antibody titre in sera of chicken.
After challenge test the percentage of protection were decreased and this decrease varies according to the increase of aflatoxin. The percentage of protection were 100%, 73.3%, 66.6%, 60%, 53.4%, 86.7%, 80% and 0.0% in group 1, 2, 3, 4, 5, 6, 7, and 8 respectively.
For studied the interaction between aflatoxin Candida
albicans, one day old chicks were divided into 5 groups and
each group divided into A and B where group A recieved aflatoxin only and group B received aflatoxin and infected with Candida albicans. Group 1 A and B fed normal feed,
II group 2 A and 8 fed 125 ppb/kg feed, group 3 A and 8 fed 250
ppb/kg feed, group 4 A and 8 fed 500 ppb/kg and group 5 A and 8 fed 1000 ppb/kg.
Resistance of chicken to Candida albicans were measured by clinical sign, post mortem, mycological by isolation of Candida albicans and histopathological examination.
We found resistance of chicken were decreased and this decreased varies according to the increase of aflatoxin. The percentage of infectivity in crop and proventroulous in groups 18, 2B, 3B, 4B, and 58 were 26.6% , 66.6%, 73.3%, 86.6% and 93.3% respectively. While in groups lA, 2A, 3A, 4A and 5A were 0%.
In the third experiment, one day old chicks were divided into A and 8 where group A received aflatoxin only and group B received aflatoxin and infected with Aspergillus fumigatus.
Group 1 A and 8 normal feed, group 2 A and 8 fed 125 ppb/kg feed, group 3 A and 8 fed 250 ppb/kg group 4 A and 8 fed 1000 ppb/kg feed.
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| Publication year |
1992
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| Availability location |
7 شارع نادى الصيد - الدقى - الجيزة(المعمل المركزى لتحليل متبقيات المبيدات والعناصر الثقيلة فى الأغذية)
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| Availability number |
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| Organization Name |
Central Laboratory of Residue Analysis of Pesticides & Heavy Metals in Foods (QCAP) Accredited according to ISO 17025
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| Country |
Egypt
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| Department |
Microbiology Analysis
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Newcastle disease virus.
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| Proposed Agrovoc |
Chicks--Diseases--Prevention;
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| Publication Type |
Master Thesis
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