| Titles |
|
English :
|
Studies on freezing of rabbit semen.
|
|
Arabic :
|
دراسات على تجميد السائل المنوى في الارانب.
|
|
| Abstract |
The present study was carried out in the Rabbitry Farm, Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, Egypt, during the period from April to December, 2000.Twenty buck and one-hundred twenty nine New-Zealand White (NZW) multiparous lactating doe rabbits aged 12-20 month and 3.2-3.6 kg body weight, were used in the present study.
Tow experiments were carried out. The first experiment aimed to study the effects of different extenders (tris-yolk fructose, lactose-yolk citrate or sucrose-yolk citrate) and levels of either glycerol or DMSO (2,4 or 6%) on the percentages of post-thawing motility, freezability and acrosomal damage of the thawed-frozen rabbit spermatozoa, during thawing-incubation at 37oC for up to 2 hours. The second experiment was carried out to investigate the effect of packaging method (straws or as pellets) and caffeine concentrations (0,2.5,5 and 10mM) of the thawed- frozen rabbit semen on the percentages of post-thawing motility, freezability, acrosomal damage of spermatozoa, fructose content and fructolysis index, during thawing-incubation at 37oC for up to 2 hours. Enzymatic activities (GOT, GPT, ALP, ACP and LDH) of the thawed-frozen rabbit semen, were estimated. The kindling rate and litter size at birth for does artificially inseminated using frozen semen either in straws or as pellets supplemented with or without 5mM caffeine, were also assessed.
The results obtained were as follows:
Experiment 1:
1. Sucrose-yolk citrate and tris-yolk fructose extenders were significantly (P<0.01) better than lactose-yolk citrate extender in maintaining thawed-frozen good rabbit semen quality, during thawing-incubation at 370C for 2 hours.
2. The 2% glycerol or 4% DMSO maintained significantly (P<0.01) higher percentages of post-thawing motility, freezability and acrosomal integrity of frozen rabbit spermatozoa as compared with the other levels of glycerol or DMSO.
3. The incubation time at 370C for 2 hours of the thawed-frozen buck rabbits semen decreased significantly (P<0.01) the percentages of post-thawing motility and freezability and increased significantly (P<0.01) the percentage of spermatozoa with damaged acrosomes.
Experiment 2:
1. Frozen-thawed semen quality
1.1. The percentages of post-thawing motility and freezability of buck rabbits spermatozoa frozen in straws were significantly (P<0.01) higher and significantly (P<0.01) lower the percentage of acrosomal damage than those frozen as pellets form.
1.2. The 5mM caffeine added to the thawed-frozen buck rabbits semen either in straws or as pellets significantly (P<0.01) higher the percentages of post-thawing motility, freezability and fructolysis index of rabbit spermatozoa while, significantly (P<0.01) lower the percentage of acrosomal damage of spermatozoa and fructose content than free caffeine medium.
1.3. The incubation time at 370C for 2 hours of the thawed-frozen buck rabbit semen decreased significantly (P<0.01) the percentages of post-thawing motility, freezability and fructose content of buck rabbit spermatozoa while, increased significantly (P<0.01) the percentage of acrosomal damage and fructolysis index in frozen-semen either in straws or as pellets.
2. Enzymatic activities
2.1. Frozen-semen in straws showed insignificant lower the amounts of glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactic dehydrogenase (LDH) released into the extracellular medium than frozen-semen as pellets form, during thawing-incubation at 37oC for up to 2 hours.
2.2. The 5 mM caffeine added to the thawed-semen either in straws or as pellets significantly (P<0.01) lower the amounts of GOT, GPT, ALP, ACP and LDH released into the extracellular medium than free caffeine medium, during thawing-incubation at 37oC for up to 2 hours.
2.3. Incubation time of the thawed-frozen rabbits semen either in straws or as pellets at 37oC for up to 2 hours increased significantly (P<0.01) the amount of GOT, GPT, ALP, ACP and LDH released into the extracellular medium.
3. The kindling rate and litter size at birth for does artificially inseminated with the thawed frozen-semen in straws and supplemented with 5mM caffeine were insignificantly higher than as pellets form or free-caffeine medium.
In conclusion, frozen-rabbits semen in straws supplemented with 5mM caffeine is more efficient in maintaining good thawed-semen quality and enzymatic activities than as pellets form or free caffeine medium. Also, frozen-semen should be inseminated soon post-thawing to avoid any sperm damage. The technique of frozen-semen as pellets form using paraffin-wax showed almost the same results of kindling rate and litter size at birth as straws. Therefore, it could be recommended to use the pelleting technique supplemented with 5mM caffeine by employing paraffin-wax for packaging semen especially under the developing countries conditions which could solve the problems of high cost of the imported straws or lack of dry ice to improve the fertility when used for artificial insemination.
Key words: Semen collection, frozen-semen, straws, pellets.
|
| Publication year |
2002
|
| Availability location |
مكتبة معهد بحوث الانتاج - شارع نادى الصيد- الدقى - الجيزة
|
| Availability number |
887
|
| Organization Name |
Animal Production Research Institute (APRI)
|
| Country |
Egypt
|
| Department |
Biotechnolog Research Department
|
| Author(s) from ARC |
|
| Agris Categories |
Animal physiology and biochemistry
|
AGROVOC
TERMS |
Animal physiology.
|
| Publication Type |
Master Thesis
|