Titles |
English :
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Incidence of mycoplasma infection in chicken farms in matrouh governorate by using recent techniques.
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Arabic :
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تواجد عدوى الميكوبلازما فى الدجاج فى محافظة مطروح باستخام التقنيات الحديثة.
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Abstract |
One hundred and eighteen chicken tracheal swabs as well as serum samples were col lected from different flocks at Matrouh and Sidi Brani ELISA positive reactions were 36.44 % for Mycoplasma gailisepticum (MG) and 8.47 % for Mycoplasma synoviae (MS).
The suitability of.polyrnerase chain reaction (PCR) for the detection of either Mycoplas ma gallisepticum (MG) or M. synoviae (MS) was compared with cultural method. The cu! ture was positive for 8.47 % of the examined swabs, while 12.7! % of the samples were found to be positive by PCR.
Intraspecies genotypic heterogeneity among isolates and strains of Mycoplasma galli. septicum and M. synoviae was demonstrated with the arbitrarily primed polymerase chaifl reaction (AP-PCR) method. The differences according to the banding patterns of their am plified DNA on agarose gels were characteristic for specific isolates. The method can be used to determine ihe similarity between isolates of MG and MS from various sources
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Publication year |
2001
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Availability location |
مكتبة معهد بحوث صحة الحيوان 7 ش نادى الصيد- الدقى0
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Availability number |
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Organization Name |
Animal Health Research Institute (AHRI)
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City |
القاهرة
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serial title |
مجلة الجمعية المصرية الطبية البيطرية
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Department |
Mycoplasma
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Author(s) from ARC |
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Agris Categories |
Animal diseases
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AGROVOC TERMS |
Chickens.
DNA.
Elisa.
Infection.
Mycoplasma.
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Proposed Agrovoc |
incidence;
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Publication Type |
Journal
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