| Titles |
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English :
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Direct detection of mycoplasma gallIseplcum (S6 strain) by polymerase chain reaction (pcr)
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Arabic :
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إستخدام سلسلة تفاعل أنزيم البوليمريز للكشف السريع للميكوبلازما جاليسبتكم عترة S6
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| Abstract |
The polymerase chain reaction was used for rapid detection and differentiation between different strains of Mycoplasma gallisepticuin. Two oligonucleotide primers of 22 and 23 base pair in length were constructed. The PCR amplification products were visualized by ethidluin bromide and ultraviolet exposure. The results were as follows: 1. A positive si PCR product 960 bp in size was detected for Mycoplasma gallisepticum (S6 strains).
2. Other strains of mycoplasma (orale, arginini, salivarium and pneumonia no band at 960 bp molecular weight. This technique provides a simple and extremely sensitive method of identifying isolates of M. gallisepticum and other Mycoplasmas.
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| Publication year |
1995
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| Availability location |
مكتبة معهد بحوث صحة الحيوان 7 ش نادى الصيد- الدقى0
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| Availability number |
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| Organization Name |
Animal Health Research Institute (AHRI)
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| City |
القاهرة
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| serial title |
المجلة المصرية للبحوث الزراعية
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| Department |
Mycoplasma
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Mycoplasma gallisepticum.
PCR.
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| Publication Type |
Journal
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