Titles |
English :
|
Direct detection of mycoplasma gallisepicum S6 strain
by polymerase chain reaction, PCR
|
Arabic :
|
إستخدام سلسلة تفاعل أنزيم البوليمريز للكشف السريع للميكوبلازما جاليسبتكم عترة S6
|
|
Abstract |
The polymerase chain reaction was used for rapid detection and differentiation between different strains of Mycoplasma gallisepticuim Two oligonucleotide primers of 22 and 23 base pair in length were constructed. The PCR amplification products were visualized by ethidluim bromide and ultraviolet exposure. The results were as follows: 1. A positive single PCR product 960 bp in size was detected for Mycoplasma gallisepticum (S6 strains).
2. Other strains of mycoplasma (orale, arginini, salivarium and pneumonia no band at 960 bp molecular weight. This technique provides a simple and extremely sensitive method of identifying isolates of M. gallisepticum and other Mycoplasmas.
|
Publication year |
1995
|
Organization Name |
Animal Health Research Institute (AHRI)
|
Country |
Egypt
|
City |
أسيوط
|
serial title |
المجلة الطبية البيطرية بأسيوط
|
Volume |
23
. 64
|
Department |
Mycoplasma
|
Author(s) from ARC |
|
Agris Categories |
Animal diseases
|
AGROVOC TERMS |
Bromides.
Identification.
Isolation techniques.
Mycoplasma gallisepticum.
PCR.
|
Proposed Agrovoc |
polymerase enzyme; fast detection;
|
Publication Type |
Journal
|