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English :
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Studies of ethanolic extract from lentinus edodes on different cell lines And lymphocytes separated from cancer breast patients
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Arabic :
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دراسات على تأثير المستخلص الايثانولى لمشروم الشيتاكى علىDifferent cell lines and lymphocytes المفصولة من مرضى سرطان الثدى
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| Abstract |
Lentinus edodes a Chinese edible and medicinal mushroom was grown using submerged culture on different standard liquid media and different food wastes by products. Shiitake broth medium was the medium that showed the maximum biomass for both tested strains. The biomass obtained from cultivation on all tested media was extracted with ethanol to obtain crude extract that tested for its biological activity. Also, SM broth medium showed the highest crude extract yield for the two strains. Some physiological factors were studied to optimize the conditions for cultivation on SM broth medium for maximum biomass and crude extract yield. Although recent studies have demonstrated that L. edodes separate compounds can inhibit the proliferation of cancer cells In vitro and in vivo, crude extract of L. edodes mycelia was tested in this study In vitro and in vivo. The In vitro cytotoxic activity of the crude extracts of mycelia of the two strains were examined on human breast cancer (MCF7) cell line using trypan blue exclusion assayu and MTT cytotoxicity assay. The results showed that the crude extract of Lentinus edodes LC2141 inhibited the proliferation of MCF7 at high concentration with IC50 178.8 µg/ml while Lentinus edodes LC202 extract inhibited the proliferation of MCF7 at high con centration with IC50 94.025 µg / ml.Also, The treatment of Hep-G2 cells with different concentrations of ethanolic extract of both Lentinus edodes LC2141 and Lentinus edodes LC202 dramatically inhibited the cell growth in a dose-dependent manner, with high IC50 values of 953.1 µg / ml and 1868.3 µg / ml, respectively. Immunomodulating activity of the tested extracts was determined on normal and breast cancer patient Iymphocyte using MTT and trypan blue exclusion assays. The treatment of Iymphocyte with different concentration of the crude extract showed an increase in Iymphocyte proliferation by increasing concentration (dose dependant).
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| Publication year |
2011
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| Organization Name |
Food Technology Research Institute (FTRI)
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| serial title |
Cancer Biology.
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| Author(s) from ARC |
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| Publication Type |
Journal
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